Exner T, Rickard K A, Speers S
Haemostasis. 1977;6(3):157-62. doi: 10.1159/000214175.
Plasma from a patient factor VIII inhibitor was mixed at several low concentrations with fresh normal plasma. The factor VIII deficient plasmas obtained after a short incubation period were used as substrate plasmas in the normal one-stage factor VIII assay method. Results obtained using 0.5-1% of the inhibitor plasma in normal plasma compared favourably with those obtained using normal haemophilia A factor VIII deficient plasma provided that tests were carried out without delay. A single plasma exchange of this patient provided enough antibody to prepare more than 500 litres of factor VIII deficient plasma by simple mixing with fresh normal plasma.
将一名患有因子VIII抑制剂患者的血浆与新鲜正常血浆按几种低浓度混合。短时间孵育后获得的因子VIII缺乏血浆用作正常一步法因子VIII测定方法中的底物血浆。如果立即进行检测，在正常血浆中使用0.5 - 1%的抑制剂血浆所获得的结果与使用正常甲型血友病因子VIII缺乏血浆所获得的结果相当。该患者单次血浆置换所提供的抗体，通过与新鲜正常血浆简单混合，足以制备超过500升的因子VIII缺乏血浆。
