Department of Hygiene and Oral Health, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Neurosci Res. 2010 Jul;67(3):228-35. doi: 10.1016/j.neures.2010.03.007. Epub 2010 Mar 17.
In the present study we examined the effects of neonatal orosensory deprivation on taste-elicited gustatory activity in the rat parabrachial nucleus (PBN) using the functional anatomical marker c-Fos. Animals in three groups (GG, GO and GM) received gastric cannula implantation surgery on postnatal day 9 (P9). Animals in the fourth group (MR) did not receive any surgery. GG rats were fed by infusion of artificial milk directly into the stomach. GO rats were fed by intraoral infusion of artificial milk. GM and MR rats were reared by their mother with free access to mother's milk, water and rat chow. Rats from all groups were similar in body weight and length by P21. On P21 rats in all groups were intraorally presented with 0.5M sucrose solution and the brains were extracted and processed for c-Fos immunohistochemistry. Taste-elicited c-Fos expression in both the gustatory waist area, and the external lateral subnucleus of the PBN in rats in the GG group was significantly more robust than in the other three groups. These findings suggest a substantial alteration in orosensory-evoked neuronal response in this nucleus, due to sensory or motor deprivation during a critical developmental stage.
在本研究中，我们使用功能性解剖标记物 c-Fos 研究了新生期口感觉剥夺对大鼠臂旁核（PBN）味觉诱发味觉活动的影响。三组动物（GG、GO 和 GM）在出生后第 9 天（P9）接受胃管植入手术。第四组动物（MR）未接受任何手术。GG 大鼠通过将人工奶直接注入胃中来喂养。GO 大鼠通过口腔内输注人工奶来喂养。GM 和 MR 大鼠由其母亲喂养，可自由饮用母乳、水和大鼠饲料。到 P21 时，所有组的大鼠体重和体长相似。在 P21 时，所有组的大鼠均经口给予 0.5M 蔗糖溶液，提取大脑并进行 c-Fos 免疫组织化学分析。与其他三组相比，GG 组大鼠 PBN 的味觉腰区和外侧外亚核中味觉诱发的 c-Fos 表达明显更为强烈。这些发现表明，由于在关键发育阶段的感觉或运动剥夺，该核中的口感觉诱发神经元反应发生了实质性改变。
