Takeda H, Chang C
Department of Surgery/Urology, University of Chicago, Illinois 60637.
J Endocrinol. 1991 Apr;129(1):83-9. doi: 10.1677/joe.0.1290083.
Ontogeny of the androgen receptor in the mouse prostate during fetal and post-natal periods was re-evaluated by histochemistry using a monoclonal antibody and by in-situ hybridization using a cDNA probe. Unlike steroid autoradiographic techniques which show ligand-binding sites in autoradiographs, these two techniques make it possible to investigate the expression of receptor protein and the transcription of its mRNA directly at the cellular level. Our previous autoradiographic data have demonstrated that only the mesenchyme takes up androgen during the fetal period; no incorporation of androgen by the epithelium has been observed in the fetus, although this does occur in later development. This observation indicated that the morphogenesis of prostatic epithelium is induced by the androgen-activated surrounding mesenchyme. In the post-natal prostate, the present data are nearly the same as those obtained by autoradiographic techniques; ligand-binding activity and expression of the receptor and its mRNA are much higher in the epithelium than in the stroma. On the other hand, the present study indicated that the fetal epithelium, which had shown no ligand-binding activity by autoradiographic analysis, expressed the same amount of receptor protein and its mRNA as the fetal mesenchyme. The results suggest that androgen receptor protein is expressed in the fetal epithelium but may not be able to bind to androgens until post-natal development.
利用单克隆抗体通过组织化学方法以及使用cDNA探针通过原位杂交技术，对小鼠前列腺在胎儿期和出生后雄激素受体的个体发生进行了重新评估。与在放射自显影片中显示配体结合位点的类固醇放射自显影技术不同，这两种技术能够直接在细胞水平研究受体蛋白的表达及其mRNA的转录。我们之前的放射自显影数据表明，在胎儿期只有间充质摄取雄激素；在胎儿中未观察到上皮细胞摄取雄激素，尽管在后期发育中确实会发生这种情况。这一观察结果表明前列腺上皮的形态发生是由雄激素激活的周围间充质诱导的。在出生后的前列腺中，目前的数据与通过放射自显影技术获得的数据几乎相同；上皮细胞中受体及其mRNA的配体结合活性和表达远高于基质。另一方面，本研究表明，通过放射自显影分析未显示配体结合活性的胎儿上皮，其表达的受体蛋白及其mRNA的量与胎儿间充质相同。结果表明，雄激素受体蛋白在胎儿上皮中表达，但在出生后发育之前可能无法与雄激素结合。
