Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, 5700 San Luis, Argentina.
J Steroid Biochem Mol Biol. 2010 May;120(1):45-52. doi: 10.1016/j.jsbmb.2010.03.040. Epub 2010 Mar 18.
The ovarian nervous plexus (ONP) is one of the principal extrinsic innervation pathways reaching the ovary from the superior mesenteric ganglion (SMG). The aims of this work were: (a) to determine if acetylcholine (Ach) in the SMG modifies the release of steroids and ovarian nitrites in an ex vivo SMG-ONP-ovary system on dioestrus (D) I and II, and (b) to demonstrate if the activities and gene expression of the steroidogenic enzymes 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) are modified by cholinergic stimulus. The system was incubated in Krebs-Ringer buffer bicarbonate at 37 degrees C in metabolic bath. Ach (10(-6)M) was used as cholinergic agonist. Ach in SMG increased progesterone release at all the incubation times on DI and DII (*p<0.001). Androstenedione increased at 15 and 30min on DI, and at 30min on DII whereas nitric oxide (NO) increased at 30min on DI, and at 15 and 30min on DII. The activity of 3beta-HSD increased whereas the activity of 20alpha-HSD decreased (*p<0.001) on DI and DII. The gene expression of 3beta-HSD showed a significant increase at 120min on DI and DII ((o)p<0.01) and 20alpha-HSD diminished only on DII. The results show the importance of the SMG via the ovarian nervous plexus on the regulation of the steroid secretory activity and on the ovarian release of NO in the luteal phase. The complex synaptic connections in the prevertebral ganglia and the sympathetic ganglionic chain participate in the neuroendocrinological mechanisms that take place during the luteal steroidogenesis.
卵巢神经丛（ONP）是从肠系膜上神经节（SMG）到达卵巢的主要外在神经支配途径之一。本工作的目的是：（a）确定 SMG 中的乙酰胆碱（Ach）是否在发情期（D）I 和 II 的体外 SMG-ONP-卵巢系统中改变类固醇和卵巢亚硝酸盐的释放，以及（b）证明类固醇生成酶 3β-羟甾脱氢酶（3β-HSD）和 20α-羟甾脱氢酶（20α-HSD）的活性和基因表达是否受胆碱能刺激的影响。该系统在 37°C 的代谢浴中在碳酸氢盐 Krebs-Ringer 缓冲液中孵育。Ach（10（-6）M）用作胆碱能激动剂。SMG 中的 Ach 在 DI 和 DII 的所有孵育时间均增加孕酮释放（*p<0.001）。雄烯二酮在 DI 上增加 15 和 30min，在 DII 上增加 30min，而一氧化氮（NO）在 DI 上增加 30min，在 DII 上增加 15 和 30min。3β-HSD 的活性增加，而 20α-HSD 的活性降低（*p<0.001）在 DI 和 DII 上。3β-HSD 的基因表达在 DI 和 DII 的 120min 时显著增加（（o）p<0.01），而 20α-HSD 仅在 DII 上减少。结果表明，SMG 通过卵巢神经丛在黄体期调节类固醇分泌活性和卵巢 NO 释放方面具有重要意义。椎前神经节和交感神经节链中的复杂突触连接参与黄体类固醇生成过程中的神经内分泌机制。
