Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, 5.700 San Luis, Argentina.
Steroids. 2010 Oct;75(10):685-94. doi: 10.1016/j.steroids.2010.04.005. Epub 2010 Apr 28.
Both peripheral innervation and nitric oxide (NO) participate in ovarian steroidogenesis. The aims of the work were (1) to investigate whether ganglionic noradrenergic (NE) and cholinergic (Ach) stimulus modify the ovarian steroids and NO release and (2) to examine the effect of those stimuli on the mRNA expression of 3beta-HSD and P450 aromatase in the ovary. The experiments were carried out using the ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system of rats in the first oestral cycle. The system was incubated in a buffer solution for 120min, with the ganglion and ovary located in different compartments and linked by the SON. NE and Ach were added into the ganglion compartment. Both NE and Ach predominantly induced ovarian release of androstenedione and oestradiol while inhibited progesterone release. Ovarian NO release increased after ganglionic stimulation during proestrous while its secretion decreased during the diestrous. Noteworthily, 3beta-HSD and P450 aromatase expression were modulated by neural stimulation. In the follicular phase, Ach in CG increased 3beta-HSD and NE increased P450 aromatase. In the luteal phase both neurotransmitters increased 3beta-HSD and Ach increased P450 aromatase transcript levels. All above observations suggest that the preponderancy of an either noradrenergic or cholinergic effect would depend on the stage of the first oestral cycle in the rat. The ovarian response to noradrenergic and cholinergic stimuli on GC, via SON, is strongly linked to oestral-stage-specific ovarian structures and their secretion products.
外周神经支配和一氧化氮（NO）都参与卵巢甾体生成。本研究的目的是：（1）探讨神经节去甲肾上腺素（NE）和胆碱能（Ach）刺激是否改变卵巢甾体和 NO 释放；（2）研究这些刺激对卵巢 3β-HSD 和 P450 芳香化酶 mRNA 表达的影响。实验采用第一动情周期大鼠腹腔神经节-卵巢上神经-卵巢（CG-SON-O）离体系统进行。该系统在缓冲液中孵育 120min，神经节和卵巢位于不同隔室，由 SON 连接。将 NE 和 Ach 添加到神经节隔室中。NE 和 Ach 主要诱导卵巢分泌雄烯二酮和雌二醇，而抑制孕酮释放。促黄体激素期卵巢神经刺激后 NO 释放增加，而在动情前期其分泌减少。值得注意的是，神经刺激调节卵巢 3β-HSD 和 P450 芳香化酶的表达。在卵泡期，CG 中的 Ach 增加 3β-HSD，而 NE 增加 P450 芳香化酶。在黄体期，两种神经递质均增加 3β-HSD，Ach 增加 P450 芳香化酶转录水平。所有这些观察结果表明，NE 或 Ach 的优势作用取决于大鼠动情周期的阶段。通过 SON，卵巢对 GC 中去甲肾上腺素和胆碱能刺激的反应与动情期特异性卵巢结构及其分泌产物密切相关。
