Center for Environmental Research and Technology, Chemical and Environmental Engineering Department, Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA 92507, USA.
Bioresour Technol. 2010 Aug;101(15):5941-51. doi: 10.1016/j.biortech.2010.03.003. Epub 2010 Mar 20.
Lignin in pretreated cellulosic biomass can non-productively adsorb cellulase, resulting in loss of a significant portion of this expensive protein. In addition, lignin interferes with the path for cellulase action, slowing down hydrolysis. Thus, the effectiveness of enzymatic hydrolysis of pretreated lignocellulosic biomass can be significantly enhanced if lignin is removed or effectively modified before adding enzymes. In this study, the enzymatic digestibilities of solids resulting from using the surfactants Tween-80, dodecylbenzene sulfonic acid, and polyethylene glycol 4000 during water-only or dilute acid pretreatment of corn stover at 140-220 degrees C were evaluated. All of these surfactants increased lignin removal during pretreatment and reduced non-productive binding of enzymes on the biomass surface, but Tween-80 increased enzymatic hydrolysis yields and enhanced total sugar recovery more than the other two. Surfactant pretreatment was found to improve lignin solubility, which could improve cellulose digestibility by reducing unproductive binding to enzyme, and also appeared to enhance performance by modifying the biomass surface.
预处理纤维素生物质中的木质素会非生产性地吸附纤维素酶，导致这种昂贵蛋白质的很大一部分损失。此外，木质素会干扰纤维素酶作用的途径，从而减缓水解速度。因此，如果在添加酶之前去除或有效修饰木质素，则可以显著提高预处理木质纤维素生物质的酶解效率。在这项研究中，评估了在 140-220°C 下仅用水或稀酸预处理玉米秸秆时使用吐温 80、十二烷基苯磺酸和聚乙二醇 4000 这三种表面活性剂对固体的酶消化率。所有这些表面活性剂都增加了预处理过程中的木质素去除率，并减少了酶在生物质表面的非生产性结合，但吐温 80 比其他两种表面活性剂提高了酶解产率和总糖回收率。表面活性剂预处理被发现可以提高木质素的溶解度，这可以通过减少酶的非生产性结合来提高纤维素的消化率，并且似乎还通过修饰生物质表面来提高性能。
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