表面活性剂辅助的碱性预处理和酶水解以在降低酶负载量的情况下提高糖回收率。
Surfactant-assisted alkaline pretreatment and enzymatic hydrolysis of for enhancing sugar recovery with a reduced enzyme loading.
作者信息
Cheng Xiyu, Luo Ying, Gao Yifan, Li Shen, Xu Chunming, Tang Shangyuan, Yang Yongkun, Zhang Zehua, Jiang He, Xu Hanli, Shi Shuobo, Yan Qiong
机构信息
College of Life Sciences and Bioengineering, School of Physical Science and Engineering, Beijing Jiaotong University, Beijing, China.
Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing, China.
出版信息
Front Bioeng Biotechnol. 2022 Jul 22;10:918126. doi: 10.3389/fbioe.2022.918126. eCollection 2022.
Surfactants play a vital role in the delignification and saccharification of lignocellulosic biomass. A strategy for coupling surfactant-assisted alkaline pretreatment (SAP) with surfactant-assisted enzymatic hydrolysis (SEH) has been proposed for improving sugar recovery from a potential energy crop, . Poly (ethylene glycol) 2000 (PEG 2000) was found to be more efficient in SAP than in other tested surfactants. Compositional and structural analysis revealed that the SAP process with 1% of PEG 2000 produced more efficient lignin removal and microstructure disruption of the pretreated sample, thus indicating much higher reducing sugar yields of 544.4-601.2 mg/g compared to the samples that were untreated or pretreated by alkali alone. Moreover, SEH with 1% Tween 80, which could block the lignin-enzyme interactions, produced a substantial reduction of 33.3% in the enzyme loading to achieve a higher sugar recovery from the SAP sample.
表面活性剂在木质纤维素生物质的脱木质素和糖化过程中起着至关重要的作用。为了提高从一种潜在能源作物中回收糖分的效率，人们提出了一种将表面活性剂辅助碱性预处理（SAP）与表面活性剂辅助酶水解（SEH）相结合的策略。研究发现，聚乙二醇2000（PEG 2000）在SAP过程中比其他测试的表面活性剂更有效。成分和结构分析表明，使用1% PEG 2000的SAP工艺能更有效地去除木质素，并破坏预处理样品的微观结构，因此与未处理或仅用碱预处理的样品相比，还原糖产量显著提高，达到544.4 - 601.2毫克/克。此外，使用1%吐温80进行SEH，可阻断木质素与酶的相互作用，使酶用量大幅减少33.3%，从而从SAP样品中获得更高的糖回收率。
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