Szentner Kinga, Waśkiewicz Agnieszka, Imbiorowicz Robert, Borysiak Sławomir
Department of Chemistry, Faculty of Forestry and Wood Technology, Poznan University of Life Sciences, Wojska Polskiego 75, 60625 Poznan, Poland.
Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland.
Molecules. 2024 Dec 7;29(23):5785. doi: 10.3390/molecules29235785.
In recent years, many studies have focused on improving the bioconversion of cellulose by adding non-ionic surfactants. In our study, the effect of the addition of a polymer, polyethylene glycol (PEG 4000), on the bioconversion of different cellulose materials was evaluated, focusing on the hydrolysis efficiency and structural changes in pure cellulose after the enzymatic hydrolysis process. The obtained results showed that the addition of non-ionic surfactant significantly improved the digestibility of cellulosic materials. The highest hydrolysis efficiency was observed for Sigmacel 101 (Cel-S101) cellulose, which consists mainly of amorphous regions. In the case of Avicel cellulose (Cel-A), PEG had a lesser effect on the bioconversion's efficiency due to limited access to the crystal structure and limited substrate-cellulase interactions. The consistency of the obtained results is confirmed by qualitative and quantitative analyses (XRD, FTIR, and HPLC). Our findings may be helpful in further understanding the mechanism of the action of surfactants and improving the enzymatic hydrolysis process.
近年来，许多研究都集中在通过添加非离子表面活性剂来提高纤维素的生物转化效率。在我们的研究中，评估了添加聚合物聚乙二醇（PEG 4000）对不同纤维素材料生物转化的影响，重点关注酶水解过程后纯纤维素的水解效率和结构变化。所得结果表明，添加非离子表面活性剂显著提高了纤维素材料的消化率。对于主要由无定形区域组成的Sigmacel 101（Cel-S101）纤维素，观察到了最高的水解效率。在微晶纤维素（Cel-A）的情况下，由于对晶体结构的可及性有限以及底物与纤维素酶的相互作用有限，PEG对生物转化效率的影响较小。通过定性和定量分析（XRD、FTIR和HPLC）证实了所得结果的一致性。我们的研究结果可能有助于进一步理解表面活性剂的作用机制并改进酶水解过程。
