Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Am J Surg Pathol. 2010 May;34(5):620-6. doi: 10.1097/PAS.0b013e3181d73293.
This study was undertaken to elucidate the genetic patterns of the renal cell neoplasms of oncocytosis and to compare them with those found in cases with multiple oncocytomas. Three cases of renal oncocytosis and 6 cases of multiple oncocytomas were analyzed. Fluorescence in situ hybridization analysis was performed with centromeric probes for chromosomes 1, 2, 6, 10, and 17 that are typically lost in chromophobe renal cell carcinoma but not in oncocytoma. Immunohistochemistry for cytokeratin 7, parvalbumin, and S100A1 was performed in all cases. Eleven tumors were present in the 3 kidneys with oncocytosis. One of these was a classic chromophobe renal cell carcinoma. In the other 10, none showed any chromosomal losses, whereas 3 showed gains of all 5 chromosomes and 1 had gains of chromosomes 2 and 10. The chromophobe renal cell carcinoma showed losses of chromosome 1, 6, 10, and 17. Twelve of 14 tumors from the patients with multiple oncocytomas showed no loss of any of the chromosomes 1, 2, 6, 10, or 17 and 2 had loss of chromosome 1. All the tumors from kidneys with renal oncocytosis showed strong parvalbumin immunoreactivity, whereas cytokeratin 7 and S100A1 expression were variable. In summary, the renal cell neoplasms of oncocytosis seem to have distinct morphologic, immunohistochemical, and cytogenetic profiles and likely are a distinct entity, not closely related to oncocytoma or chromophobe renal cell carcinoma.
这项研究旨在阐明肾嗜酸细胞瘤的遗传模式，并将其与多发性嗜酸细胞瘤病例的遗传模式进行比较。分析了 3 例肾嗜酸细胞瘤和 6 例多发性嗜酸细胞瘤。应用针对染色体 1、2、6、10 和 17 着丝粒的探针进行荧光原位杂交分析，这些染色体在嫌色细胞肾细胞癌中通常丢失，但不在嗜酸细胞瘤中丢失。对所有病例进行细胞角蛋白 7、副甲状腺球蛋白和 S100A1 的免疫组织化学染色。在 3 例有嗜酸细胞瘤的肾脏中发现了 11 个肿瘤。其中一个是典型的嫌色细胞肾细胞癌。在另外 10 个肿瘤中，没有任何一个显示任何染色体丢失，而 3 个肿瘤显示所有 5 条染色体的增益，1 个肿瘤显示染色体 2 和 10 的增益。嫌色细胞肾细胞癌显示染色体 1、6、10 和 17 的丢失。14 个多发性嗜酸细胞瘤患者的肿瘤中有 12 个没有任何染色体 1、2、6、10 或 17 的丢失，2 个有染色体 1 的丢失。所有来自有肾嗜酸细胞瘤的肾脏的肿瘤均显示强烈的副甲状腺球蛋白免疫反应性，而细胞角蛋白 7 和 S100A1 的表达则不同。总之，肾嗜酸细胞瘤似乎具有独特的形态、免疫组织化学和细胞遗传学特征，可能是一种独特的实体，与嗜酸细胞瘤或嫌色细胞肾细胞癌没有密切关系。
