大鼠肝脏缺血再灌注中的后适应效应
Postconditioning effect in the hepatic ischemia and reperfusion in rats.
作者信息
Santos Carlos Henrique Marques dos, Pontes José Carlos Dorsa Vieira, Miiji Luciana Nakao Odashiro, Nakamura Diogo Iengo, Galhardo Cesar Augusto Vendas, Aguena Sandra Maura
机构信息
Surgery Department, UFMS, Mato Grosso do Sul, Brazil.
出版信息
Acta Cir Bras. 2010 Apr;25(2):163-8. doi: 10.1590/s0102-86502010000200008.
PURPOSE
To evaluate the effect of postconditioning protection in liver tissue of rats submitted to ischemia and reperfusion.
METHODS
25 Wistar male rats were randomized in three groups: Group A (ischemia and reperfusion - I/R), with 10 rats, which was made ischemia by vascular clamp application in hepatic hilum for 30 minutes and reperfusion for 60 minutes by removal of the clamp; Group B (Postconditioning - IPo), with 10 rats, with same procedure plus postconditioning (3 cycles of reperfusion inserted by 3 cycles of ischemia, 30 seconds for each phase, between ischemia and reperfusion phases); and Group C (Sham), with 5 rats, which took place only laparotomy and manipulation of the hepatic hilum. Specimens were examined (histological evaluation) and dosage of serum AST and ALT was made. The statistical analysis was made with t Student test, with significant difference when p<0.05.
RESULTS
In the Sham group there was no histological or enzymatic changes; In group A the mean tissue lesion was 1.6 and in group B 1.1 (p=0.014). In group A the mean ALT level was 355U/l and in group B was 175.9U/l (p=0.016). The mean of AST was 828.8U/l in group A and 295.5U/l in group B (p=0.001).
CONCLUSION
The postconditioning was able to minimize the severity of liver injury in rats submitted to ischemia and reperfusion.
目的
评估后适应保护对经历缺血再灌注的大鼠肝脏组织的影响。
方法
25只雄性Wistar大鼠随机分为三组：A组（缺血再灌注组 - I/R），共10只大鼠，通过夹闭肝门血管30分钟造成缺血，然后移除夹子再灌注60分钟；B组（后适应组 - IPo），共10只大鼠，采用相同程序并加用后适应处理（在缺血和再灌注阶段之间插入3个缺血/再灌注循环，每个阶段30秒，共3个循环）；C组（假手术组），共5只大鼠，仅进行剖腹术和肝门操作。对标本进行检查（组织学评估）并测定血清AST和ALT水平。采用t检验进行统计学分析，p<0.05时有显著差异。
结果
假手术组无组织学或酶学变化；A组平均组织损伤为1.6，B组为1.1（p = 0.014）。A组平均ALT水平为355U/l，B组为175.9U/l（p = 0.016）。A组AST平均值为828.8U/l，B组为295.5U/l（p = 0.001）。
结论
后适应能够减轻经历缺血再灌注的大鼠肝脏损伤的严重程度。
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