Santos Carlos Henrique Marques dos, Aydos Ricardo Dutra, Nogueira Neto Ed, Miiji Luciana Nakao Odashiro, Cassino Pedro Carvalho, Alves Isadora Ishaq, Calheiros Nádia Meneguesso, Garcia Milena
Department of Surgery, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil.
Department of Pathology, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil.
Braz J Cardiovasc Surg. 2015 Sep-Oct;30(5):533-7. doi: 10.5935/1678-9741.20150067.
Some publications have demonstrated the presence of lung reperfusion injury in mesenteric ischemia and reperfusion (I/R), but under to diverse methods. Postconditioning has been recognized as effective in preventing reperfusion injury in various organs and tissues. However, its effectiveness has not been evaluated in the prevention of lung reperfusion injury after mesenteric ischemia and reperfusion.
To evaluate the presence of pulmonary reperfusion injury and the protective effect of ischemic postconditioning on lung parenchyma in rats submitted to mesenteric ischemia and reperfusion.
Thirty Wistar rats were distributed into three groups: group A (10 rats), which was held mesenteric ischemia (30 minutes) and reperfusion (60 minutes); group B (10 rats), ischemia and reperfusion, interspersed by postconditioning with two alternating cycles of reperfusion and reocclusion, for two minutes each; and group C (10 rats), ischemia and reperfusion interleaved by postconditioning with four alternating cycles of reperfusion and reocclusion of 30 seconds each. Finally, it was resected the upper lung lobe for histological analysis.
There were mild lung lesions (grade 1) in all samples. There was no statistical difference between groups 1 and 2 (P >0.05).
The mesenteric ischemia and reperfusion in rats for thirty and sixty minutes, respectively, caused mild reperfusion injury in lung. Postconditioning was not able to minimize the remote reperfusion injury and there was no difference comparing two cycles of two minutes with four cycles of 30 seconds.
一些出版物已证实在肠系膜缺血再灌注（I/R）中存在肺再灌注损伤，但采用的方法各不相同。缺血后处理已被认为对预防各种器官和组织的再灌注损伤有效。然而，其在预防肠系膜缺血再灌注后的肺再灌注损伤方面的有效性尚未得到评估。
评估大鼠肠系膜缺血再灌注后肺再灌注损伤的存在情况以及缺血后处理对肺实质的保护作用。
30只Wistar大鼠分为三组：A组（10只大鼠），进行肠系膜缺血（30分钟）和再灌注（60分钟）；B组（10只大鼠），缺血和再灌注，期间穿插进行两个交替的再灌注和再闭塞循环，每次两分钟的后处理；C组（10只大鼠），缺血和再灌注，期间穿插进行四个交替的再灌注和再闭塞循环，每次30秒的后处理。最后，切除上肺叶进行组织学分析。
所有样本均有轻度肺损伤（1级）。1组和2组之间无统计学差异（P>0.05）。
大鼠分别进行30分钟和60分钟的肠系膜缺血再灌注，导致肺部轻度再灌注损伤。缺血后处理未能将远处的再灌注损伤降至最低，两分钟的两个循环与30秒的四个循环相比没有差异。
