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1993-2008 年斯洛文尼亚侵袭性流感嗜血杆菌分离株的特征。

Characterisation of invasive Haemophilus influenzae isolates in Slovenia, 1993-2008.

机构信息

Department of Medical Microbiology, National Institute of Public Health, Grabloviceva 44, 1000, Ljubljana, Slovenia.

出版信息

Eur J Clin Microbiol Infect Dis. 2010 Jun;29(6):661-8. doi: 10.1007/s10096-010-0910-6. Epub 2010 Mar 21.

Abstract

The objectives of our study were to describe the epidemiology of invasive Haemophilus influenzae disease from 1993 to 2008 in Slovenia, a country with routine H. influenzae serotype b (Hib) conjugate vaccination since the year 2000. A total of 292 isolates of H. influenzae, recovered from a normally sterile site, were collected in the study period. The isolates were serotyped by slide agglutination and antibiotic susceptibility was determined. One hundred and eight isolates received after the year 2000 were serotyped by slide agglutination and by polymerase chain reaction (PCR) capsule typing, and both methods were compared. After the introduction of the routine Hib vaccination, the incidence of H. influenzae disease in children under the age of 5 years has decreased by 87.6% and type b was replaced by non-typeable H. influenzae as the predominant serotype. The proportion of serotype b decreased from 85.3% in the pre-vaccination period to 13.0% in the vaccination period and the proportion of non-capsulated isolates increased from 12.0 to 85.2%. The study of genetic relatedness by pulsed-field gel electrophoresis (PFGE) demonstrated that the isolates of serotypes b and f were genetically homogeneous within the serotype. The results of our national surveillance showed that the vaccine has been very efficient in preventing Hib invasive disease in Slovenia. Nevertheless, we see a need for further monitoring of invasive H. influenzae infections at a national level.

摘要

本研究的目的是描述 1993 年至 2008 年期间斯洛文尼亚侵袭性流感嗜血杆菌病的流行病学情况,该国自 2000 年以来常规接种乙型流感嗜血杆菌(Hib)结合疫苗。在研究期间,共收集了 292 株从正常无菌部位分离出的流感嗜血杆菌分离株。通过玻片凝集法对分离株进行血清分型,并测定抗生素敏感性。2000 年后获得的 108 株分离株通过玻片凝集法和聚合酶链反应(PCR)荚膜分型进行血清分型,并比较了这两种方法。常规 Hib 疫苗接种后,5 岁以下儿童侵袭性流感嗜血杆菌病的发病率下降了 87.6%,b 型被无荚膜流感嗜血杆菌取代成为主要血清型。b 型的比例从疫苗接种前的 85.3%下降到疫苗接种期间的 13.0%,无荚膜分离株的比例从 12.0%增加到 85.2%。脉冲场凝胶电泳(PFGE)的遗传相关性研究表明,b 型和 f 型的分离株在血清型内具有遗传同源性。我们的国家监测结果表明,该疫苗在斯洛文尼亚预防 Hib 侵袭性疾病非常有效。然而,我们认为有必要在国家层面进一步监测侵袭性流感嗜血杆菌感染。

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