Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019, USA.
Rapid Commun Mass Spectrom. 2010 Apr 30;24(8):1113-23. doi: 10.1002/rcm.4489.
In previous studies, new electrospray ionization mass spectrometry (ESI-MS) approaches were developed for the highly sensitive detection of singly and doubly charged anions in positive mode ESI-MS by using specially synthesized dicationic and tricationic ion-pairing agents, respectively. By detecting the positively charged ion complex in the positive mode, limits of detection (LODs) for the anions can be lowered by several magnitudes. In this work, we used eighteen newly synthesized tetracationic ion-pairing agents, constructed with different geometries, linkages and cation moieties, for the detection of eighteen triply charged anions of different structural motifs. The LODs for these anions were from ten to several thousand times lower in the positive selective ion monitoring (SIM) mode than in the negative mode. These tetracationic agents also were shown to be useful for the detection of -1 and -2 anions. In addition, the LODs for -3 anions can be further lowered by monitoring the product fragments of the ion-pairing complexes in the single reaction monitoring (SRM) mode.
在以前的研究中，分别开发了新的电喷雾电离质谱（ESI-MS）方法，使用专门合成的二价和三价离子对试剂，分别用于正模式 ESI-MS 中单电荷和双电荷阴离子的高灵敏度检测。通过在正模式下检测带正电荷的离子配合物，可以将阴离子的检测限（LOD）降低几个数量级。在这项工作中，我们使用了十八种新合成的四价离子对试剂，它们具有不同的几何形状、连接和阳离子部分，用于检测十八种不同结构模式的三价阴离子。与负离子模式相比，这些负离子在正选择性离子监测（SIM）模式下的检测限（LOD）低了十个到几千个数量级。这些四价试剂还可用于检测-1 和-2 价阴离子。此外，通过在单反应监测（SRM）模式下监测离子对配合物的产物碎片，可以进一步降低-3 价阴离子的检测限。
