载脂蛋白 E 基因型和饮食槲皮素对 apoE3 和 apoE4 转基因小鼠对氧磷酶 1 状态的影响。
Impact of apolipoprotein E genotype and dietary quercetin on paraoxonase 1 status in apoE3 and apoE4 transgenic mice.
机构信息
Institute of Human Nutrition and Food Science, Christian-Albrechts-University, Kiel, Germany.
出版信息
Atherosclerosis. 2010 Jul;211(1):110-3. doi: 10.1016/j.atherosclerosis.2010.02.027. Epub 2010 Feb 26.
OBJECTIVES
The aim of the present study was to determine hepatic paraoxonase 1 (PON1) status in response to apoE genotype and dietary quercetin supplementation in mice.
METHODS AND RESULTS
ApoE3 and apoE4 transgenic mice were fed semi-synthetic diets without (controls) and with quercetin (2 mg/g diet) for 6 weeks. Hepatic mRNA and protein levels of PON1 were significantly lower in apoE4 as compared to apoE3 mice. Feeding quercetin-enriched diets induced hepatic PON1 gene expression with a tendency for greater induction in apoE3 as compared to apoE4 mice. Furthermore, hepatic mRNA and protein levels of beta-glucuronidase and sulfatase, both enzymes centrally involved in the deconjugation of quercetin conjugates, were lower in apoE4 vs. apoE3 mice. PPARgamma (which partly controls PON1 gene expression) mRNA levels were lower in apoE4 vs. apoE3 mice.
CONCLUSION
We provide first evidence that PON1 is differentially regulated in response to apoE genotype.
目的
本研究旨在确定载脂蛋白 E 基因型和饮食补充槲皮素对小鼠肝过氧化物酶 1（PON1）状态的影响。
方法和结果
apoE3 和 apoE4 转基因小鼠分别喂食不含（对照）和含槲皮素（2mg/g 饮食）的半合成饮食 6 周。与 apoE3 小鼠相比，apoE4 小鼠的肝 PON1mRNA 和蛋白水平显著降低。富含槲皮素的饮食诱导肝 PON1 基因表达，apoE3 小鼠的诱导作用强于 apoE4 小鼠。此外，与 apoE3 小鼠相比，apoE4 小鼠中β-葡萄糖醛酸酶和磺基转移酶的肝 mRNA 和蛋白水平较低，这两种酶均参与槲皮素结合物的去结合。apoE4 小鼠的过氧化物酶体增殖物激活受体 γ（部分控制 PON1 基因表达）mRNA 水平低于 apoE3 小鼠。
结论
我们首次提供证据表明，PON1 对载脂蛋白 E 基因型的反应存在差异调节。
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