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CAAT/增强子结合蛋白能够与ATF/CRE元件结合。

CAAT/enhancer binding protein is able to bind to ATF/CRE elements.

作者信息

Bakker O, Parker M G

机构信息

Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, London, UK.

出版信息

Nucleic Acids Res. 1991 Mar 25;19(6):1213-7. doi: 10.1093/nar/19.6.1213.

DOI:10.1093/nar/19.6.1213
PMID:2030941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC333845/
Abstract

The transcription factor C/EBP is known to be able to bind to two different classes of sites, a CAAT-box and one present in a number of viral enhancers. In this paper we show using band-shift assays, methylation interference and footprinting that C/EBP is also able to bind with high affinity to ATF/CRE sites. Competition with mutant ATF sites and methylation interference indicate that C/EBP may be able to bind to the ATF/CRE sites by virtue of their homology to the enhancer core elements. Furthermore, we show that C/EBP is able to direct transcription from this site in transient transfection experiments and that mutations in the ATF binding site that impair DNA binding also effect the ability of C/EBP to stimulate transcription.

摘要

已知转录因子C/EBP能够结合两类不同的位点，即CAAT盒以及存在于多种病毒增强子中的一种位点。在本文中，我们通过凝胶迁移试验、甲基化干扰和足迹法表明，C/EBP还能够以高亲和力结合ATF/CRE位点。与突变型ATF位点的竞争和甲基化干扰表明，C/EBP可能凭借其与增强子核心元件的同源性而能够结合到ATF/CRE位点。此外，我们表明在瞬时转染实验中C/EBP能够指导从此位点进行转录，并且ATF结合位点中损害DNA结合的突变也会影响C/EBP刺激转录的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/b5efb90bf476/nar00242-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/209f544f5772/nar00242-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/27a9bb5b5cd5/nar00242-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/f0913235c0e5/nar00242-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/b5efb90bf476/nar00242-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/209f544f5772/nar00242-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/27a9bb5b5cd5/nar00242-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/f0913235c0e5/nar00242-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/accd/333845/b5efb90bf476/nar00242-0047-a.jpg

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本文引用的文献

1
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
2
Firefly luciferase gene: structure and expression in mammalian cells.萤火虫荧光素酶基因：在哺乳动物细胞中的结构与表达
Mol Cell Biol. 1987 Feb;7(2):725-37. doi: 10.1128/mcb.7.2.725-737.1987.
3
Tissue-specific in vitro transcription from the mouse albumin promoter.来自小鼠白蛋白启动子的组织特异性体外转录。
白色3T3-L1和棕色HIB-1B前脂肪细胞中Pgc-1α启动子上环状AMP反应元件的组合转录因子调控
J Biol Chem. 2009 Jul 31;284(31):20738-52. doi: 10.1074/jbc.M109.021766. Epub 2009 Jun 2.
4
Regulation of the steroidogenic acute regulatory protein gene expression: present and future perspectives.类固醇生成急性调节蛋白基因表达的调控：现状与未来展望。
Mol Hum Reprod. 2009 Jun;15(6):321-33. doi: 10.1093/molehr/gap025. Epub 2009 Mar 25.
5
Role of basic leucine zipper proteins in transcriptional regulation of the steroidogenic acute regulatory protein gene.碱性亮氨酸拉链蛋白在类固醇生成急性调节蛋白基因转录调控中的作用。
Mol Cell Endocrinol. 2009 Apr 10;302(1):1-11. doi: 10.1016/j.mce.2008.12.009. Epub 2008 Dec 25.
6
Transcriptional activation of the small GTPase gene rhoB by genotoxic stress is regulated via a CCAAT element.基因毒性应激对小GTP酶基因rhoB的转录激活作用是通过一个CCAAT元件来调控的。
Nucleic Acids Res. 2001 Feb 1;29(3):792-8. doi: 10.1093/nar/29.3.792.
7
Amino acids control mammalian gene transcription: activating transcription factor 2 is essential for the amino acid responsiveness of the CHOP promoter.氨基酸调控哺乳动物基因转录：激活转录因子2对CHOP启动子的氨基酸反应性至关重要。
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8
ATF3 and stress responses.激活转录因子3与应激反应
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9
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10
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J Virol. 1993 Oct;67(10):6192-200. doi: 10.1128/JVI.67.10.6192-6200.1993.
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4
A multiplicity of CCAAT box-binding proteins.多种CCAAT框结合蛋白。
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5
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Anal Biochem. 1986 Jul;156(1):251-6. doi: 10.1016/0003-2697(86)90180-6.
6
The thyroid hormone receptor binds with opposite transcriptional effects to a common sequence motif in thyroid hormone and estrogen response elements.甲状腺激素受体与甲状腺激素和雌激素反应元件中的一个共同序列基序结合，产生相反的转录效应。
Cell. 1988 Jul 29;54(3):313-23. doi: 10.1016/0092-8674(88)90194-8.
7
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Biochemistry. 1987 Nov 17;26(23):7234-8. doi: 10.1021/bi00397a006.
8
Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.反式作用蛋白因子与真核转录调控：来自DNA肿瘤病毒研究的经验教训
Genes Dev. 1988 Mar;2(3):267-81. doi: 10.1101/gad.2.3.267.
9
Interactions of purified transcription factors: binding of yeast MAT alpha 1 and PRTF to cell type-specific, upstream activating sequences.纯化转录因子的相互作用：酵母MATα1和PRTF与细胞类型特异性上游激活序列的结合
EMBO J. 1988 Dec 20;7(13):4255-64. doi: 10.1002/j.1460-2075.1988.tb03323.x.
10
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Proc Natl Acad Sci U S A. 1988 May;85(10):3396-400. doi: 10.1073/pnas.85.10.3396.