Isachenko Vladimir, Isachenko Evgenia, Weiss Juergen M, Todorov Plamen, Kreienberg Rolf
Department of Obstetrics and Gynaecology, University of Ulm, Prittwitzstrasse 43,89075 Ulm, Germany.
Cryo Letters. 2009 Nov-Dec;30(6):449-54.
The first case of cryopreservation of human ovarian tissue with good survival of follicles after warming was described in 1996. Childbirth after cryopreservation of ovarian tissue is now a reality. Cryopreservation of ovarian tissue can be performed using one of two methods: conventional ("slow") freezing and cryopreservation by direct plunging into liquid nitrogen (so called vitrification or "rapid" freezing). Comparative investigations of vitrification and conventional freezing performed on mammalian ovarian tissue are limited, and authors present different conclusions. The higher effectiveness of vitrification in comparison with conventional freezing for human oocytes and embryos was shown, whereas data on human ovarian tissue are limited. The aim of different studies was to compare the safety and effectiveness of conventional freezing and vitrification of human ovarian tissue. Below we shortly summarize the results of some investigations with different conclusions. The discussion on the post-warming quality of follicles as well as on the problems of microbial contamination of cells in liquid nitrogen at vitrification is presented. In our opinion, for cryopreservation of human ovarian tissue, conventional freezing is more promising than vitrification.
1996年报道了首例人类卵巢组织冷冻保存且复温后卵泡存活良好的病例。卵巢组织冷冻保存后分娩如今已成为现实。卵巢组织冷冻保存可采用两种方法之一：传统的（“慢速”）冷冻和直接投入液氮进行冷冻保存（即所谓的玻璃化或“快速”冷冻）。对哺乳动物卵巢组织进行的玻璃化和传统冷冻的比较研究有限，且作者得出了不同的结论。已表明玻璃化相对于传统冷冻对人类卵母细胞和胚胎更有效，而关于人类卵巢组织的数据有限。不同研究的目的是比较人类卵巢组织传统冷冻和玻璃化的安全性和有效性。下面我们简要总结一些得出不同结论的研究结果。还讨论了复温后卵泡的质量以及玻璃化时液氮中细胞的微生物污染问题。我们认为，对于人类卵巢组织的冷冻保存，传统冷冻比玻璃化更具前景。
