Marks J R, Davidoff A M, Kerns B J, Humphrey P A, Pence J C, Dodge R K, Clarke-Pearson D L, Iglehart J D, Bast R C, Berchuck A
Department of Surgery, Duke University Medical Center, Durham, North Carolina 22710.
Cancer Res. 1991 Jun 1;51(11):2979-84.
We examined p53 expression in 107 epithelial ovarian cancers with immunohistochemical techniques using monoclonal antibody PAb1801. High level expression of nuclear p53 protein was detected in the malignant epithelium in 54 (50%) of these cancers. Expression of p53 protein was undetectable in 13 benign gynecological tissues. p53 mRNA from three cancers that overexpressed the protein were sequenced and point mutations which altered the coding sequence of the highly conserved region of the gene were found in each case. Three cancers with undetectable protein levels also were sequenced and were found to be wild-type through the same region of the gene. As in other cancers, overexpression of the p53 protein in ovarian cancer appears to correlate closely with the presence of mutation in the p53 gene. p53 overexpression did not correlate with stage, histological grade, or the ability to perform optimal cytoreductive surgery. A significant correlation (P = 0.04) was observed between p53 overexpression and aneuploidy in advanced stage (III/IV) disease. There was no significant relationship between overall survival and p53 expression. Since mutation and overexpression of p53 are common in epithelial ovarian cancers, further studies are warranted to clarify the role of p53 in ovarian tumorigenesis.
我们使用单克隆抗体PAb1801，通过免疫组化技术检测了107例上皮性卵巢癌中的p53表达情况。在这些癌症中，有54例（50%）的恶性上皮细胞中检测到核p53蛋白的高水平表达。在13例良性妇科组织中未检测到p53蛋白的表达。对3例p53蛋白过表达的癌症的p53 mRNA进行测序，发现每例均存在改变该基因高度保守区域编码序列的点突变。对3例蛋白水平检测不到的癌症也进行了测序，发现该基因的同一区域为野生型。与其他癌症一样，卵巢癌中p53蛋白的过表达似乎与p53基因的突变密切相关。p53过表达与分期、组织学分级或进行最佳细胞减灭术的能力无关。在晚期（III/IV期）疾病中，观察到p53过表达与非整倍体之间存在显著相关性（P = 0.04）。总生存期与p53表达之间无显著关系。由于p53的突变和过表达在上皮性卵巢癌中很常见，因此有必要进一步研究以阐明p53在卵巢肿瘤发生中的作用。
