Anjorin Andrew Olushola, Olaofe Olaejirinde Olaniyi, Anjorin Atinuke Olu, Omoniyi-Esan Ganiat Olutoyin, Komolafe Akinwumi Oluwole
Department of Anatomic Pathology & Forensic Medicine, Osun State University, Osogbo, Nigeria.
Department of Morbid Anatomy & Forensic Medicine, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
BMC Womens Health. 2024 Dec 5;24(1):639. doi: 10.1186/s12905-024-03487-0.
p53 is a tumor suppressor gene. p53 expression in epithelial ovarian tumors (EOTs) is correlated with their biological behavior and predicts patient overall survival. However, there is a dearth of knowledge regarding p53 expression in these tumors among women from southwest Nigeria. Our study aimed to determine the patterns of p53 expression in various types of epithelial ovarian tumours.
We conducted a retrospective study of epithelial ovarian tumours. We retrieved formalin-fixed, paraffin-embedded (FFPE) tissue blocks of previously diagnosed epithelial tumors from the departmental archive. We performed immunohistochemical analysis using p53 antibodies. We scored the expression and staining intensity of p53 as follows: negative (0), focal/weakly positive (1 +), and diffuse/strongly positive (2 +) on the basis of the recommended Cytomation scoring system.
The spectrum of p53 expression in the 51 histologically diagnosed cases revealed that 29 cases had no expression, consisting of 21 benign EOTs, two borderline EOTs, and six malignant EOTs. Nine cases exhibited wild-type expression, including six serous carcinomas, two mucinous carcinomas, and one signet ring cell carcinoma. p53 overexpression was observed in 13 patients overall, with 12 having serous carcinomas and one having endometrioid carcinoma. Among the 21 serous carcinoma patients, 28.6% (6 patients) presented with wild-type p53 expression, 57.1% (12 patients) presented with p53 overexpression, and 14.3% (three patients) presented negative p53 expression. There was a significant association between p53 expression and the histological grade of serous carcinoma.
Most epithelial ovarian carcinomas in our hospital are high grade, with many serous carcinomas showing either p53 overexpression or loss of expression. This may contribute to the poor patient survival rate.
p53是一种肿瘤抑制基因。p53在上皮性卵巢肿瘤（EOTs）中的表达与其生物学行为相关，并可预测患者的总生存期。然而，关于尼日利亚西南部女性这些肿瘤中p53表达的知识却很匮乏。我们的研究旨在确定不同类型上皮性卵巢肿瘤中p53的表达模式。
我们对上皮性卵巢肿瘤进行了一项回顾性研究。我们从科室档案中检索了先前诊断的上皮性肿瘤的福尔马林固定、石蜡包埋（FFPE）组织块。我们使用p53抗体进行免疫组化分析。我们根据推荐的Cytomation评分系统对p53的表达和染色强度进行如下评分：阴性（0）、局灶性/弱阳性（1+）和弥漫性/强阳性（2+）。
在51例组织学诊断的病例中，p53表达谱显示29例无表达，包括21例良性EOTs、2例交界性EOTs和6例恶性EOTs。9例表现为野生型表达，包括6例浆液性癌、2例黏液性癌和1例印戒细胞癌。总体上在13例患者中观察到p53过表达，其中12例为浆液性癌，1例为子宫内膜样癌。在21例浆液性癌患者中，28.6%（6例）表现为野生型p53表达，57.1%（12例）表现为p53过表达，14.3%（3例）表现为p53阴性表达。p53表达与浆液性癌的组织学分级之间存在显著关联。
我院大多数上皮性卵巢癌为高级别，许多浆液性癌表现为p53过表达或表达缺失。这可能导致患者生存率较低。
