Hosker H S, Corris P A
Department of Respiratory Medicine, Freeman Hospital, Newcastle upon Tyne, U.K.
Cancer Detect Prev. 1991;15(2):103-6.
A lucigenin-enhanced chemiluminescence (CL) assay was used to assess alveolar macrophage (AM) and blood monocyte (BM) function in patients with lung cancer (LC). Ten patients with LC (7-SCLC, 3-NSCLC) and ten matched controls underwent bronchoalveolar lavage, and AMs were subjected to CL with and without stimulation with latex beads. Peak CL was recorded as counts per minute (CPM)/10(3) cells/min. BM activity was similarly assessed in 17 LC patients (13-SCLC) and 17 matched controls. Peak activity of both unstimulated and latex stimulated AMs in the LC group was higher than controls. Similarly, BM activity was enhanced in LC patients compared with controls. There was no correlation between AM CL responses and disease extent, but BM function at diagnosis correlated with subsequent response to cytotoxic chemotherapy. Results indicate both local and systemic activation of the monocyte/macrophage system in LC even in patients with limited disease.
采用光泽精增强化学发光（CL）分析法评估肺癌（LC）患者的肺泡巨噬细胞（AM）和血液单核细胞（BM）功能。10例肺癌患者（7例小细胞肺癌、3例非小细胞肺癌）和10例匹配的对照者接受支气管肺泡灌洗，对AM进行有无乳胶珠刺激下的CL检测。CL峰值记录为每分钟计数（CPM）/10³细胞/分钟。对17例肺癌患者（13例小细胞肺癌）和17例匹配的对照者同样评估BM活性。肺癌组未刺激和乳胶刺激的AM的峰值活性均高于对照组。同样，与对照组相比，肺癌患者的BM活性增强。AM的CL反应与疾病范围之间无相关性，但诊断时的BM功能与随后对细胞毒性化疗的反应相关。结果表明，即使是疾病局限的肺癌患者，单核细胞/巨噬细胞系统也存在局部和全身激活。
