Winsel K, Christ R, Eckert H, Renner H, Unger U, Grollmuss H
Forschungsinstitut für Lungenkrankheiten und Tuberkulose Berlin-Buch/DDR.
Z Erkr Atmungsorgane. 1987;169(1):23-32.
The phagocytic activity of alveolar macrophages (AM) in a group of patients with stage I, stage II and stage III pulmonary sarcoidosis (gradation according to Wurm) has been investigated. Additionally a classification in different forms of the course of sarcoidosis was made (acute = Loefgren's syndrome, latent = primary-chronic, and relapses). Patients with other lung diseases and healthy subjects were recruited as control group. The phagocytic activity (stimulation with opsonized yeast cell wall particles) of AM, which were isolated by bronchoalveolar lavage, was determined by means of chemiluminescence (CL)-measuring using lucigenin and luminol, respectively, as amplifiers. The investigations showed that the lucigenin-dependent yeast cell wall-induced CL of AM in patients with sarcoidosis is significantly increased in comparison to the control group. No significant changes of the luminol-dependent CL of AM from sarcoidosis patients could be detected. The lucigenin-dependent CL-response of AM is obviously an indicator of the intensity of the alveolitis and thus of the activity of the pathological process in sarcoidosis. The results suggest that in pulmonary sarcoidosis there is a hyperreactive AM/lymphocytes-system.
对一组I期、II期和III期肺结节病患者（根据武尔姆分级）的肺泡巨噬细胞（AM）的吞噬活性进行了研究。此外，还对结节病病程的不同形式进行了分类（急性=勒夫格伦综合征，潜伏性=原发性慢性，以及复发）。招募患有其他肺部疾病的患者和健康受试者作为对照组。通过支气管肺泡灌洗分离出的AM的吞噬活性（用调理素化酵母细胞壁颗粒刺激），分别使用光泽精和鲁米诺作为放大器，通过化学发光（CL）测量来确定。研究表明，与对照组相比，结节病患者中AM的光泽精依赖性酵母细胞壁诱导的CL显著增加。未检测到结节病患者AM的鲁米诺依赖性CL有显著变化。AM的光泽精依赖性CL反应显然是肺泡炎强度的一个指标，因此也是结节病病理过程活性的指标。结果表明，在肺结节病中存在AM/淋巴细胞系统的高反应性。
