Waalewijn R A, Meuwissen S G, Pals G, Hoefsmit E C
Department of Gastroenterology, Free University Hospital, Amsterdam/The Netherlands.
Eur J Cell Biol. 1991 Feb;54(1):55-60.
The localization of pepsinogens (PG A and PG C) was studied intracellularly in human gastric biopsies embedded in Lowicryl K4M, using affinity-purified antibodies and protein A-gold. The homogeneous secretory granules of the chief cells contained both PG A and PG C, as was proved by serial sections. Identical reaction was also seen in the core of the biphasic mucous neck cell granules, whereas the mantle did not label. The rough endoplasmic reticulum (RER) and Golgi complex of the chief cells and mucous neck cells contained ample label. Transitional cells identified by the presence of granules of both chief cells and mucous neck cells were recognized. This type of mucous neck cell is thought to transform into a chief cell. However, an increase of RER that could explain an increase of the pepsinogen production was not observed. A mixture of these granules was also found in cells morphologically characterized as young parietal cells, suggesting a common precursor for these three cell types. These observations make the transformation from mucous neck to chief cells questionable. Antral gland cells contained only PG C, as was shown in serial section, too.
采用亲和纯化抗体和蛋白A-金，在包埋于Lowicryl K4M的人胃活检组织中对细胞内胃蛋白酶原（PGA和PGC）进行定位研究。通过连续切片证实，主细胞的均质分泌颗粒同时含有PGA和PGC。在双相性黏液颈细胞颗粒的核心也观察到相同反应，而其外层未标记。主细胞和黏液颈细胞的粗面内质网（RER）和高尔基体含有大量标记物。识别出了通过同时存在主细胞和黏液颈细胞颗粒而鉴定的过渡细胞。这种类型的黏液颈细胞被认为会转化为主细胞。然而，未观察到可解释胃蛋白酶原产量增加的RER增加情况。在形态学上被鉴定为年轻壁细胞的细胞中也发现了这些颗粒的混合物，提示这三种细胞类型有共同的前体。这些观察结果使从黏液颈细胞向主细胞的转化受到质疑。连续切片也显示，胃窦腺细胞仅含有PGC。
