Meuwissen S G, Mullink H, Bosma A, Pals G, Défize J, Flipse M, Westerveld B D, Tas M, Brakké J, Kreuning J
Prog Clin Biol Res. 1985;173:185-97.
An immunoperoxidase method using antisera specific for PG I and PG II was developed to assess PG-containing cells in the human stomach. Fundus-chief cells contained abundant PG I and less PG II. Mucus neck cells stained more frequently with anti-PG I, while antral gland cells stained only with anti-PG II. In adenocarcinoma of the corpus, isolated glands faintly staining with anti-PG I or anti-PG II were observed. In dense tumorous tissue no anti-PG I activity could be observed, while PG II sometimes remained present in residual antral glands, even in areas of heavy tumor infiltration. Focal PG II activity was also clearly demonstrated in some antral tumor cells. The immunoperoxidase technique described is suitable for further detailed studies of normal and pathological conditions of the gastric mucosa.
开发了一种使用针对胃蛋白酶原I（PG I）和胃蛋白酶原II（PG II）的特异性抗血清的免疫过氧化物酶方法，以评估人胃中含PG的细胞。胃底主细胞含有丰富的PG I，而PG II较少。黏液颈细胞用抗PG I染色更频繁，而胃窦腺细胞仅用抗PG II染色。在胃体腺癌中，观察到孤立的腺体用抗PG I或抗PG II染色微弱。在致密的肿瘤组织中未观察到抗PG I活性，而PG II有时仍存在于残留的胃窦腺体中，即使在肿瘤浸润严重的区域也是如此。在一些胃窦肿瘤细胞中也清楚地显示出局灶性PG II活性。所描述的免疫过氧化物酶技术适用于对胃黏膜正常和病理状况的进一步详细研究。
