来自马铃薯的一种腺苷三磷酸双磷酸酶同工酶对合成焦磷酸酯的水解作用。
Hydrolysis of synthetic pyrophosphoric esters by an isoenzyme of apyrase from Solanum tuberosum.
作者信息
Del Campo G, Puente J, Valenzuela M A, Traverso-Cori A, Cori O
出版信息
Biochem J. 1977 Dec 1;167(3):525-9. doi: 10.1042/bj1670525.
Abstract
A highly purified isoenzyme of apyrase obtained from potatoes (Solanum tuberosum var. Pimpernel) exhibits a low specificity for the organic moiety of synthetic pyro- and triphosphates. Methyl di- and tri-phosphates were hydrolysed at higher rates than ADP and ATP, but their Km values were also higher. Steric hindrance at the carbon atom linked to the pyrophosphate chain decreases both binding and maximum rate, whereas length or polarity of the organic chain do not have systematic effects. t-Butyl diphosphate, inorganic pyrophosphate, adenosine 5'-[alpha,beta-methylene]triphosphate and adenosine 5'-[beta,gamma-methylene]triphosphate are competitive inhibitors of the hydrolysis of ATP and ADP.
摘要
从马铃薯（Solanum tuberosum var. Pimpernel）中获得的一种高度纯化的腺苷三磷酸双磷酸酶同工酶，对合成焦磷酸和三磷酸的有机部分表现出低特异性。甲基二磷酸和三磷酸的水解速率高于二磷酸腺苷（ADP）和三磷酸腺苷（ATP），但其米氏常数（Km值）也更高。与焦磷酸链相连的碳原子处的空间位阻会降低结合力和最大反应速率，而有机链的长度或极性则没有系统性影响。叔丁基二磷酸、无机焦磷酸、腺苷5'-[α,β-亚甲基]三磷酸和腺苷5'-[β,γ-亚甲基]三磷酸是ATP和ADP水解的竞争性抑制剂。
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本文引用的文献
1
2
The apyrase activity of potato extract.马铃薯提取物的腺苷三磷酸双磷酸酶活性。
Arch Biochem Biophys. 1962 Jun;97:609-10. doi: 10.1016/0003-9861(62)90131-5.
3
Effects of a phosphonic acid analog of adenosine triphosphate on actomyosin systems.三磷酸腺苷的膦酸类似物对肌动球蛋白系统的影响。
Arch Biochem Biophys. 1960 Jun;88:183-92. doi: 10.1016/0003-9861(60)90220-4.
4
CONFORMATION CHANGES AT THE ACTIVE SITE DURING ENZYME ACTION.酶促反应过程中活性位点的构象变化。
Fed Proc. 1964 May-Jun;23:719-26.
5
Studies on apyrases.关于腺苷三磷酸双磷酸酶的研究。
Arch Biochem Biophys. 1961 May;93:353-63. doi: 10.1016/0003-9861(61)90278-8.
6
Studies on thiamine triphosphate.三磷酸硫胺素的研究。
Biochim Biophys Acta. 1956 May;20(2):293-8. doi: 10.1016/0006-3002(56)90288-8.
7
The enzymatic hydrolysis of gamma-phenylpropyl di- and triphosphates.γ-苯丙基二磷酸和三磷酸的酶促水解
J Am Chem Soc. 1966 Apr 5;88(7):1511-3. doi: 10.1021/ja00959a035.
8
Effects of protein-modifying reagents on an isoenzyme of potato apyrase.蛋白质修饰试剂对马铃薯焦磷酸酶一种同工酶的影响。
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9
Different molecular forms of potato apyrase.马铃薯焦磷酸酶的不同分子形式。
Arch Biochem Biophys. 1970 Mar;137(1):133-42. doi: 10.1016/0003-9861(70)90420-0.
10
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