来自鹰嘴豆（鸡豆）根的膜结合ATP二磷酸水解酶的纯化与特性分析
Purification and characterization of a membrane-bound ATP diphosphohydrolase from Cicer arietinum (chick-pea)roots.
作者信息
Vara F, Serrano R
出版信息
Biochem J. 1981 Sep 1;197(3):637-43. doi: 10.1042/bj1970637.
Abstract
Microsomal membranes from Cicer arietinum (chick-pea) roots contained an ATP phosphohydrolase activity that could be solubilized by high-ionic-strength media. The enzyme has been purified to homogeneity by affinity and ion-exchange chromatography. It has the properties of an ATP diphosphohydrolase (apyrase, EC 3.6.1.5) that hydrolyses different nucleoside di- and tri-phosphates but has no activity towards monophosphoric esters and pyrophosphate. No stimulation by K+ could be demonstrated for either the membrane-bound or the purified enzyme, and therefore it would seem not to be related to the K+ -dependent ATPase postulated to mediate K+ transport in plants.
摘要
鹰嘴豆根的微粒体膜含有一种ATP磷酸水解酶活性，该活性可被高离子强度介质溶解。通过亲和色谱和离子交换色谱已将该酶纯化至同质。它具有ATP二磷酸水解酶（焦磷酸酶，EC 3.6.1.5）的特性，可水解不同的核苷二磷酸和三磷酸，但对单磷酸酯和焦磷酸无活性。无论是膜结合酶还是纯化酶，均未显示出K+的刺激作用，因此它似乎与推测介导植物中K+转运的K+依赖性ATP酶无关。
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