Wang Ming-juan, Liu Ming, Liu Wei
Institute of Pathology, Basic Medical College, Lanzhou University, Lanzhou.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Nov;29(11):1009-11.
To study the in vivo anti-tumor effect of Oxytropis kansuensis alkaloid fraction (OKAF) in live cancer cell line H22 and its influence on immune function in KM mice.
Sixty mice with transplanted mouse originated liver cancer cell line H22 were divided randomly into five groups, the three OKAF groups were administrated with OKAF in low (8 mg/kg), moderate (16 mg/kg) and high (32 mg/kg) dosage, the negative control group was administrated with normal saline 20 mL/kg and the positive control with 5-FU 15 mg/kg, respectively, once a day for 10 successive days. Then the average tumor weight (TW), tumor inhibition rate (TIR), spleen index (SI), and thymus index (TI) in them were calculated, and the spleen cell proliferation rate (SCPR) was measured by MTT assay.
TIR of low, moderate and high dosage of OKAF was 23%, 29% and 43% respectively. SI and TI in all the three OKAF groups were higher than those in the negative control group, and the two indexes in the moderate and ligh dosage OKAF groups were higher than those in the positive control group (P < 0.05). SCPR in OKAF groups at 24 h, 48 h and 72 h were higher than those in both two control groups at the same time.
OKAF shows inhibitory action on liver cancer cell line H22 tumor in KM mice, and it could also enhance the immune function of mice.
研究甘肃棘豆生物碱部位（OKAF）对肝癌细胞系H22在体的抗肿瘤作用及其对KM小鼠免疫功能的影响。
将60只移植小鼠肝癌细胞系H22的小鼠随机分为5组，3个OKAF组分别给予低（8 mg/kg）、中（16 mg/kg）、高（32 mg/kg）剂量的OKAF，阴性对照组给予20 mL/kg生理盐水，阳性对照组给予15 mg/kg 5-氟尿嘧啶，均每日1次，连续给药10天。然后计算各组小鼠的平均瘤重（TW）、抑瘤率（TIR）、脾指数（SI）和胸腺指数（TI），并用MTT法检测脾细胞增殖率（SCPR）。
低、中、高剂量OKAF的TIR分别为23%、29%和43%。3个OKAF组的SI和TI均高于阴性对照组，中、高剂量OKAF组的这两个指标高于阳性对照组（P<0.05）。OKAF组在24 h、48 h和72 h的SCPR均高于两个对照组在同一时间的SCPR。
OKAF对KM小鼠肝癌细胞系H22肿瘤具有抑制作用，且能增强小鼠的免疫功能。
