Division of Thoracic Surgery, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Cancer Sci. 2010 May;101(5):1326-30. doi: 10.1111/j.1349-7006.2010.01530.x. Epub 2010 Feb 11.
Nectin-like molecule-5 (Necl-5) is an immunoglobulin (Ig)-like molecule that is up-regulated in many types of cancer cells. It was shown experimentally that Necl-5 enhances cell migration, proliferation, and metastasis, but its clinical significance has not been documented. The aim of this study was to observe the expression of Necl-5 in surgically resected primary lung adenocarcinomas and to investigate its clinical significance. A total of 63 surgically resected primary pulmonary adenocarcinoma tissues were investigated by immunohistochemistry for the expression of Necl-5. The relationship between expression of Necl-5 and clinicopathological features was analyzed, and the influence of Necl-5 expression on outcomes in these patients was assessed. A strong expression of Necl-5 by cancer cells was observed in 43 of the 63 tumors. The overexpression of Necl-5 by cancer cells was significantly associated with lymph node metastasis (P = 0.0398), TNM staging (P = 0.0367), and the bronchioloalveolar carcinoma ratio of tumors (P = 0.0423). Furthermore, the disease-free survival rate in patients with positive Necl-5 overexpression was significantly lower than that in patients with negative Necl-5 overexpression (P = 0.0004). Multivariate survival analysis revealed Necl-5 expression to be an independent risk factor for an unfavorable outcome (P = 0.0294). Additionally, an analysis including only the stage I cases revealed that the disease-free survival rate of the Necl-5-positive group was significantly lower than that of the Necl-5-negative group (P = 0.0192). These results indicate that Necl-5 plays a role in mediating tumor cell invasion and that the overexpression of Necl-5 in cancer cells has clinical significance for prognostic evaluation of patients with primary pulmonary adenocarcinoma.
黏附分子样细胞 5（Necl-5）是一种免疫球蛋白（Ig）样分子，在许多类型的癌细胞中上调。实验表明，Necl-5 增强了细胞迁移、增殖和转移，但它的临床意义尚未被记录。本研究的目的是观察 Necl-5 在手术切除的原发性肺腺癌中的表达，并探讨其临床意义。通过免疫组织化学方法检测 63 例手术切除的原发性肺腺癌组织中 Necl-5 的表达。分析 Necl-5 表达与临床病理特征的关系，并评估 Necl-5 表达对这些患者结局的影响。在 63 例肿瘤中，有 43 例观察到癌细胞中 Necl-5 的强表达。癌细胞中 Necl-5 的过表达与淋巴结转移（P = 0.0398）、TNM 分期（P = 0.0367）和肿瘤的细支气管肺泡癌比例（P = 0.0423）显著相关。此外，Necl-5 过表达阳性患者的无病生存率明显低于 Necl-5 过表达阴性患者（P = 0.0004）。多变量生存分析显示，Necl-5 表达是不良预后的独立危险因素（P = 0.0294）。此外，仅包括 I 期病例的分析显示，Necl-5 阳性组的无病生存率明显低于 Necl-5 阴性组（P = 0.0192）。这些结果表明，Necl-5 在介导肿瘤细胞侵袭中起作用，癌细胞中 Necl-5 的过表达对原发性肺腺癌患者的预后评估具有临床意义。
