脂肪细胞肿瘤中融合基因的特异性。

Specificity of fusion genes in adipocytic tumors.

机构信息

Department of Orthopaedics, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima 770-8503, Japan.

出版信息

Anticancer Res. 2010 Feb;30(2):661-4.

PMID:20332486

Abstract

BACKGROUND

In subsets of adipocytic tumors, specific chromosomal translocations lead to the generation of fusion genes. The high mobility group A2 (HMGA2)-lipoma preferred partner (LPP) and the reciprocal LPP-HMGA2 represent such fusion genes in lipoma, while the human translocation liposarcoma (TLS)-CCAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) and the Ewing sarcoma (EWS)-CHOP in liposarcoma. However, the specificity of these fusion genes has not been established in a variety of adipocytic tumors.

PATIENTS AND METHODS

One hundred and seventy-two cases of adipocytic tumors, comprising 98 cases of lipoma and 74 cases of liposarcoma, were analyzed for the possible expression of HMGA2-LPP, LPP-HMGA2, TLS-CHOP and EWS-CHOP fusion genes, using a reverse-transcription polymerase chain reaction method.

RESULTS

In lipoma, twenty-two cases (22.4%) were associated with either HMGA2-LPP or LPP-HMGA2, while neither TLS-CHOP nor EWS-CHOP transcript was detectable. On the contrary, in liposarcoma, neither HMGA2-LPP nor LPP-HMGA2 transcript was detectable, although twenty-five cases (33.8%) were related to either TLS-CHOP or EWS-CHOP.

CONCLUSION

HMGA2-LPP and LPP-HMGA2 were specific to lipoma, and TLS-CHOP and EWS-CHOP were specific to liposarcoma.

摘要

背景

在脂肪细胞肿瘤的亚组中，特定的染色体易位导致融合基因的产生。高迁移率族蛋白 A2（HMGA2）-脂肪瘤优先伙伴（LPP）和相互的 LPP-HMGA2 是脂肪瘤中的融合基因，而人易位性脂肪肉瘤（TLS）-CCAAT/增强子结合蛋白（C/EBP）同源蛋白（CHOP）和尤文肉瘤（EWS）-CHOP 在脂肪肉瘤中。然而，这些融合基因的特异性尚未在各种脂肪细胞肿瘤中得到证实。

患者和方法

分析了 172 例脂肪细胞肿瘤，包括 98 例脂肪瘤和 74 例脂肪肉瘤，使用逆转录聚合酶链反应方法分析 HMGA2-LPP、LPP-HMGA2、TLS-CHOP 和 EWS-CHOP 融合基因的表达情况。

结果

在脂肪瘤中，22 例（22.4%）与 HMGA2-LPP 或 LPP-HMGA2 相关，而 TLS-CHOP 和 EWS-CHOP 转录本均不可检测。相反，在脂肪肉瘤中，HMGA2-LPP 和 LPP-HMGA2 转录本均不可检测，尽管 25 例（33.8%）与 TLS-CHOP 或 EWS-CHOP 相关。