Research Centers for Gastroenterology and Liver Diseases, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Jpn J Infect Dis. 2010 Mar;63(2):136-8.
Entamoeba moshkovskii and Entamoeba dispar are impossible to differentiate microscopically from the pathogenic species Entamoeba histolytica. There are limited data on the prevalence of these commensal parasites in Iran. We utilized a single-round PCR assay to determine the prevalence of E. moshkovskii, E. dispar, and E. histolytica in stool samples from Iranian patients infected with gastrointestinal disorders. After culturing of microscopy-positive isolates and extraction of DNA, PCR was carried out to differentiate the Entamoeba isolates. Out of 3,825 stool samples examined by microscopy, 58 specimens (1.52%) were infected with E. histolytica, E. dispar, or E. moshkovskii. By PCR, 2 E. histolytica (3.45%), 53 E. dispar (91.37%), 2 E. moshkovskii (3.45%), and one mixed E. dispar/E. moshkovskii infection (1.73%) were detected. In view of the reporting of E. moshkovskii in this study in Iran and the difficulty in discriminating this ameba from two similar Entamoeba spp. by microscopy, we recommend the single-round PCR assay as an alternative tool in routine diagnosis and in epidemiological studies of amebiasis.
曼氏疟原虫和迪斯帕疟原虫在显微镜下无法与致病性的溶组织内阿米巴相区分。关于这些共生寄生虫在伊朗的流行情况，数据有限。我们利用单轮 PCR 检测法，对伊朗患有胃肠道疾病的患者粪便样本中曼氏疟原虫、迪斯帕疟原虫和溶组织内阿米巴的流行情况进行了检测。在对显微镜阳性分离物进行培养和提取 DNA 后，我们通过 PCR 对肠阿米巴进行了区分。在 3825 份经显微镜检查的粪便样本中，有 58 份（1.52%）感染了溶组织内阿米巴、迪斯帕疟原虫或曼氏疟原虫。通过 PCR 检测，发现了 2 株溶组织内阿米巴（3.45%）、53 株迪斯帕疟原虫（91.37%）、2 株曼氏疟原虫（3.45%）和 1 株迪斯帕疟原虫/曼氏疟原虫混合感染（1.73%）。鉴于本研究在伊朗报道了曼氏疟原虫的存在，并且在显微镜下很难将这种阿米巴与两种相似的肠阿米巴相区分，我们建议将单轮 PCR 检测法作为常规诊断和阿米巴病流行病学研究的替代工具。
