Raeder R, Boyle M D
Department of Microbiology, Medical College of Ohio, Toledo 43614-0008.
J Immunol Methods. 1991 Apr 25;138(2):201-9. doi: 10.1016/0022-1759(91)90168-f.
A simple, rapid two-stage competitive binding radioimmunoassay is described for detecting rheumatoid factor-like antibodies in the serum of chickens immunized with bacterial immunoglobulin binding proteins. The assay could be adapted to determine the species specificity of any rheumatoid factor-like antibody by changing the species of immobilized IgG used. This assay has important practical implications for selecting suitable antibodies for detection of bacterial immunoglobulin binding proteins leaching from affinity columns in the presence of a large molar excess of IgG, and in determining the relationship between antibodies to bacterial IgG binding proteins and rheumatoid factor production.
本文描述了一种简单、快速的两阶段竞争性结合放射免疫分析法，用于检测用细菌免疫球蛋白结合蛋白免疫的鸡血清中的类风湿因子样抗体。通过改变固定化IgG的种类，该分析法可用于确定任何类风湿因子样抗体的物种特异性。该分析法对于在存在大量摩尔过量IgG的情况下选择合适的抗体以检测从亲和柱中渗出的细菌免疫球蛋白结合蛋白，以及确定针对细菌IgG结合蛋白的抗体与类风湿因子产生之间的关系具有重要的实际意义。
