el-Naggar H M, Abou-Zakham A A, Romia S M, Hegazi M M, el-Khouly E S
Department of Parasitology, Faculty of Medicine, Mansoura University, Egypt.
J Egypt Soc Parasitol. 1991 Apr;21(1):127-34.
Large numbers of free-living stages of Ancylostoma duodenale can be grown in vitro on formalin-killed Escherichia coli supplemented with cholesterol and Dulbecco's minimum essential medium (DMEN). Eggs at 28 degrees C hatched and released first-stage larvae in 24 hr. First-stage larvae molted in 36 hr. at 28 degrees C. Second-stage larvae, molted in 2-3 days at 28 degrees C, but retained the old cuticle. Third-stage larvae characterized by the presence of a buccal cap capsule; sheath; filariform esophagus and absence of lips. Lateral alae were observed in all free-living stages.
十二指肠钩口线虫的大量自由生活阶段可在添加了胆固醇和杜氏改良伊格尔培养基（DMEM）的福尔马林灭活大肠杆菌上进行体外培养。在28摄氏度下，虫卵在24小时内孵化并释放出第一期幼虫。第一期幼虫在28摄氏度下36小时后蜕皮。第二期幼虫在28摄氏度下2至3天蜕皮，但保留了旧的角质层。第三期幼虫的特征是存在口囊；鞘；丝状食管且无唇。在所有自由生活阶段均观察到侧嵴。
