Departamento de Acuicultura, Antofagasta Institute of Renewable Resources, Universidad de Antofagasta, Campus Coloso, Antofagasta, Chile.
Microb Ecol. 2010 Aug;60(2):282-90. doi: 10.1007/s00248-010-9647-0. Epub 2010 Mar 24.
This study reports on the factors involved in regulating the composition and structure of bacterial communities epiphytic on intertidal macroalgae, exploring their temporal variability and the role of copper pollution. Culture-independent, molecular approaches were chosen for this purpose and three host species were used as models: the ephemeral Ulva spp. (Chlorophyceae) and Scytosiphon lomentaria (Phaeophyceae) and the long-living Lessonia nigrescens (Phaeophyceae). The algae were collected from two coastal areas in Northern Chile, where the main contrast was the concentration of copper in the seawater column resulting from copper-mine waste disposals. We found a clear and strong effect in the structure of the bacterial communities associated with the algal species serving as host. The structure of the bacterial communities also varied through time. The effect of copper on the structure of the epiphytic bacterial communities was significant in Ulva spp., but not on L. nigrescens. The use of 16S rRNA gene library analysis to compare bacterial communities in Ulva revealed that they were composed of five phyla and six classes, with approximately 35 bacterial species, dominated by members of Bacteroidetes (Cytophaga-Flavobacteria-Bacteroides) and α-Proteobacteria, in both non-polluted and polluted sites. Less common groups, such as the Verrucomicrobiae, were exclusively found in polluted sites. This work shows that the structure of bacterial communities epiphytic on macroalgae is hierarchically determined by algal species > temporal changes > copper levels.
本研究报告了调控附着在潮间带大型海藻上的细菌群落组成和结构的因素，探讨了它们的时间变异性和铜污染的作用。为此目的选择了非培养的、分子的方法，并使用了三种宿主物种作为模型：短暂的 Ulva spp.（绿藻）和 Scytosiphon lomentaria（褐藻）以及长寿的 Lessonia nigrescens（褐藻）。藻类是从智利北部的两个沿海地区采集的，那里的主要对比是由于铜矿废物处理导致海水中铜的浓度。我们发现，与作为宿主的藻类物种相关的细菌群落的结构有明显而强烈的影响。细菌群落的结构也随时间而变化。铜对附着细菌群落结构的影响在 Ulva spp.中是显著的，但在 L. nigrescens 中则不显著。使用 16S rRNA 基因文库分析比较 Ulva 中的细菌群落表明，它们由五个门和六个纲组成，约有 35 个细菌种，以黄杆菌-拟杆菌-acteroidetes 和α-变形菌纲的成员为主，在未污染和污染的地点都有。较不常见的群体，如 Verrucomicrobiae，仅在污染地点发现。这项工作表明，附着在大型海藻上的细菌群落的结构是由藻类物种>时间变化>铜水平层次决定的。
