College of Marine Life Sciences, Ocean University of Chinagrid.4422.0, Qingdao, China.
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
mSphere. 2022 Oct 26;7(5):e0030722. doi: 10.1128/msphere.00307-22. Epub 2022 Sep 29.
Intertidal macroalgae face periodic water loss and rehydration caused by daily tidal changes. However, the effect of water loss stress on algal epiphytic bacteria has not yet been reported. In this study, the effects of water loss stress on the epiphytic bacteria community of were analyzed, and the different responses of epiphytic bacteria to water loss stress were compared between male and female algae. The results showed that after water loss stress, the diversity of the epiphytic bacterial community of first decreased and then increased. Among the dominant taxa, the abundance of decreased significantly, whereas the abundance of and first increased and then decreased. Additionally, the indicator species and the abundance of predicted functional genes related to carbon, nitrogen, and sulfur metabolism both changed significantly. More importantly, when the epiphytic bacteria were analyzed separately according to the algal sex, the changes in algal epiphytic bacterial community structure and indicator species were more significant, and there were sexual differences. Therefore, it was concluded that water loss stress has a significant effect on the community structure and function of the epiphytic bacteria on . Meanwhile, the epiphytic bacteria community of two sexes of differed in the response to water loss stress. Periodic water loss caused by the tide is an important environmental factor that is faced by intertidal macroalgae, but the impact of periodic water loss on the epiphytic bacterial communities associated with macroalgae is still unknown. Through this study, we found that the diversity, the relative abundance of dominant taxa, the indicator species, and the abundance of the predicted functional genes in the epiphytic bacteria on changed with the time of water loss. Moreover, male and female exhibited different responses to water loss stress. This study not only paves the way for the delineation of the interactions between and its epiphytic bacteria but also provides new insights for the mechanisms of the adaptation and evolution of macroalgae in the intertidal zone.
潮间带大型海藻会周期性地经历因每日潮汐变化导致的失水和复水过程。然而，关于失水胁迫对海藻附生细菌的影响尚未见报道。在本研究中，分析了失水胁迫对 附生细菌群落的影响，并比较了雄性和雌性海藻附生细菌对失水胁迫的不同响应。结果表明，失水胁迫后， 的附生细菌群落多样性先降低后升高。在优势类群中， 的丰度显著降低，而 和 的丰度先增加后减少。此外，与碳、氮和硫代谢相关的预测功能基因的指示物种和丰度均发生显著变化。更为重要的是，当根据藻类性别对附生细菌进行单独分析时， 附生细菌群落结构和指示物种的变化更为显著，并且存在性别差异。因此，得出结论，失水胁迫对 附生细菌的群落结构和功能有显著影响。同时， 两种性别的附生细菌对失水胁迫的响应存在差异。潮水引起的周期性失水是潮间带大型海藻面临的一个重要环境因素，但周期性失水对与大型海藻相关的附生细菌群落的影响尚不清楚。通过本研究，我们发现 附生细菌的多样性、优势类群的相对丰度、指示物种和预测功能基因的丰度随失水时间而变化。此外，雄性和雌性 对失水胁迫的响应不同。本研究不仅为 及其附生细菌之间的相互作用提供了线索，也为大型海藻在潮间带的适应和进化机制提供了新的见解。
