Niño Corredor Albert Nicolás, Comba González Natalia Beatriz, Acelas Mauricio, Montoya Castaño Dolly
Group of Bioprocesses and Bioprospecting, Biotechnology Institute, Universidad Nacional de Colombia, Bogotá, Colombia.
Macromolecules Research Group, Chemistry Department, Universidad Nacional de Colombia, Bogotá, Colombia.
Biotechnol Rep (Amst). 2023 Feb 22;37:e00788. doi: 10.1016/j.btre.2023.e00788. eCollection 2023 Mar.
Marine macroalgae are being recognized as reservoirs of biologically active compounds, as their surfaces are susceptible to the colonization of microorganisms which can produce enzymes with a wide range of molecular architectures. Among these bacteria, is responsible for the biosynthesis of laccases. In this research, we performed a bioinformatic pipeline to annotate the sequenced complete genome of the epiphytic bacterium strain EPI24, from the macroalgal surface of the species; this strain showed laccase activity which has been previously assessed on plate assays. The genome of strain EPI24 has a size of ∼6.95 Mb, a GC content of 67.33%, and 6,603 protein-coding genes. The functional annotation of the strain EPI24 genome confirmed the presence of genes encoding for laccases, which could have functional properties of interest in processes such as the biodegradation of phenolic compounds under versatile and efficient conditions.
海洋大型藻类正被视为生物活性化合物的储存库，因为它们的表面易于被微生物定殖，这些微生物能够产生具有多种分子结构的酶。在这些细菌中， 负责漆酶的生物合成。在本研究中，我们执行了一个生物信息学流程，以注释从 物种的大型藻表面分离出的附生细菌 菌株EPI24的全基因组序列；该菌株已在平板试验中显示出漆酶活性。 菌株EPI24的基因组大小约为6.95 Mb，GC含量为67.33%，有6603个蛋白质编码基因。 菌株EPI24基因组的功能注释证实了编码漆酶的基因的存在，这些基因在诸如在通用且高效的条件下对酚类化合物进行生物降解等过程中可能具有令人感兴趣的功能特性。
