Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 21827, Caracas, 1020 A, Venezuela.
Parasitol Res. 2010 Apr;106(5):1225-31. doi: 10.1007/s00436-010-1798-x. Epub 2010 Mar 24.
Antigens present in aqueous n-butanolic extracts (BE) of Schistosoma mansoni (Venezuelan JL strain), Schistosoma intercalatum (Cameroon EDEA strain), and Schistosoma haematobium (Yemen strain) adult worm membranes were compared in immunoblot against sera of patients infected with S. mansoni, S. intercalatum, S. haematobium, Schistosoma japonicum, or Schistosoma mekongi looking for similarities (common antigens) and differences (species-specific antigens). About 17 S. mansoni BE polypeptides (M (r) approximately 8 to >80 kDa) were commonly recognized by S. mansoni-infected patient sera from Venezuela, Senegal, and Ethiopia. S. intercalatum-, S. haematobium-, or S. japonicum-infected sera were almost unreactive with S. mansoni BE. Nonetheless, S. mekongi-infected sera weakly cross-reacted with a approximately 10-15-kDa subset of S. mansoni BE. About 72.7% of S. intercalatum-infected patient sera reacted with a approximately 19-21-kDa complex in S. intercalatum BE and cross-reacted with a similar complex in S. haematobium BE. Conversely, all S. haematobium-infected patient sera reacted with a approximately 19-21-kDa complex in S. haematobium BE and cross-reacted with the approximately 19-21-kDa complex in S. intercalatum BE; S. mansoni- and S. japonicum-infected patient sera did not react with S. intercalatum or S. haematobium BE. Results showed the presence of a common membrane antigen between African schistosome species and species-specific antigens in S. mansoni BE that could be useful to discriminate between species and/or to detect Schistosoma infections.
用曼氏血吸虫（委内瑞拉 JL 株）、间插血吸虫（喀麦隆 EDEA 株）和埃及血吸虫（也门株）成虫膜的水正丁醇提取物（BE）中的抗原与曼氏血吸虫、间插血吸虫、埃及血吸虫、日本血吸虫或湄公血吸虫感染患者的血清进行免疫印迹比较，寻找相似性（共同抗原）和差异（种特异性抗原）。来自委内瑞拉、塞内加尔和埃塞俄比亚的曼氏血吸虫感染患者血清普遍识别约 17 种曼氏血吸虫 BE 多肽（Mr 约 8 至 >80 kDa）。间插血吸虫、埃及血吸虫或日本血吸虫感染血清与曼氏血吸虫 BE 几乎没有反应。尽管如此，湄公血吸虫感染血清与曼氏血吸虫 BE 的约 10-15 kDa 亚群弱交叉反应。约 72.7%的间插血吸虫感染患者血清与间插血吸虫 BE 中的约 19-21 kDa 复合物反应，并与埃及血吸虫 BE 中的类似复合物交叉反应。相反，所有埃及血吸虫感染患者血清均与埃及血吸虫 BE 中的约 19-21 kDa 复合物反应，并与间插血吸虫 BE 中的约 19-21 kDa 复合物交叉反应；曼氏血吸虫和日本血吸虫感染患者血清与间插血吸虫或埃及血吸虫 BE 不反应。结果表明，非洲血吸虫种之间存在共同的膜抗原，曼氏血吸虫 BE 中存在种特异性抗原，可用于区分种属和/或检测血吸虫感染。
