MSMB 基因启动子中的功能性多态性与前列腺癌风险和血清 MSMB 表达相关。

A functional polymorphism in MSMB gene promoter is associated with prostate cancer risk and serum MSMB expression.

机构信息

Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.

出版信息

Prostate. 2010 Jul 1;70(10):1146-52. doi: 10.1002/pros.21149.

DOI:10.1002/pros.21149

PMID:20333697

Abstract

BACKGROUND

To explore the reported association of SNP marker rs10993994 with prostate cancer identified by two independent in two genome-wide association studies (GWAS) further, we performed a case-control study in southern Chinese Han population. Consequently, we detected the serum levels of MSMB expression with different genotypes in the cases and controls to characterize the functional consequences of rs10993994.

MATERIALS AND METHODS

Two hundred fifty-one prostate cancer and 258 control subjects were included in the cancer association study and 90 serum samples were used to test the expression of the MSMB by Enzyme-linked immunosorbent assay (ELISA).

RESULTS

We found that the T allele displayed an increased prevalence of prostate cancer compared with the C allele (OR = 1.30, 95% CI = 1.01-1.67, P = 0.040). Moreover, the prostate cancer patients carrying CT/TT genotype had significantly decreased serum MSMB levels compared to those with CC genotype (16.32 +/- 3.98 microg/L vs. 19.33 +/- 4.27 microg/L, P = 0.022).

CONCLUSIONS

rs10993994 in MSMB promoter affects serum MSMB expression, contributes to the genetic predisposition to prostate cancer in southern Chinese Han population.

摘要

背景

为了进一步探讨两个独立的全基因组关联研究（GWAS）中 SNP 标记 rs10993994 与前列腺癌的关联，我们在汉族南方人群中进行了病例对照研究。因此，我们检测了不同基因型病例和对照者血清 MSMB 表达水平，以描述 rs10993994 的功能后果。

材料和方法

251 例前列腺癌患者和 258 例对照者纳入癌症关联研究，90 例血清样本用于通过酶联免疫吸附试验（ELISA）检测 MSMB 的表达。

结果

我们发现 T 等位基因与 C 等位基因相比，前列腺癌的患病率增加（OR=1.30，95%CI=1.01-1.67，P=0.040）。此外，与 CC 基因型相比，携带 CT/TT 基因型的前列腺癌患者血清 MSMB 水平显著降低（16.32±3.98μg/L 比 19.33±4.27μg/L，P=0.022）。