Laboratory of Clinical Microbiology, ATTIKON University Hospital, Haidari, Athens, Greece.
J Clin Lab Anal. 2010;24(2):119-22. doi: 10.1002/jcla.20352.
Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections. The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification. fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed as a suitable nucleic acid target. The gene was detected in all 17 S. lugdunensis isolates examined, while no amplification product was obtained from 98 isolates representing 11 staphylococcal and 17 nonstaphylococcal species. Forty-seven percent of the S. lugdunensis strains produced a positive slide coagulase reaction, which is consistent with varying levels of Fbl protein expression within the species.
路邓葡萄球菌是一种毒力异常的非凝固酶阴性种，与严重感染有关。本报告介绍了一种用于鉴定路邓葡萄球菌的单步、种特异性 PCR 方案。利用编码纤维蛋白原结合黏附素的 fbl 基因作为合适的核酸靶标进行了评估。该基因在检测的 17 株路邓葡萄球菌分离株中均被检出，而来自 11 种葡萄球菌和 17 种非葡萄球菌种的 98 株分离株均未获得扩增产物。47%的路邓葡萄球菌菌株产生阳性玻片凝固酶反应，这与该物种内 Fbl 蛋白表达水平的变化一致。
