Giammarinaro Philippe, Leroy Sabine, Chacornac Jean-Paul, Delmas Julien, Talon Regine
INRA, Centre de Clermont-Ferrand-Theix, UR 370, Microbiologie, 63122 Saint-Genès Champanelle, France.
J Clin Microbiol. 2005 Aug;43(8):3673-80. doi: 10.1128/JCM.43.8.3673-3680.2005.
The genus Staphylococcus is made up of 36 validated species which contain strains that are pathogenic, saprophytic, or used as starter cultures for the food industry. An oligonucleotide array targeting the manganese-dependent superoxide dismutase (sodA) gene was developed to overcome the drawbacks of the conventional methods of identification. Divergences of the sodA gene were used to design oligonucleotide probes, and we showed that each of the 36 species had a characteristic pattern of hybridization. To evaluate the array, we analyzed 38 clinical and 38 food or food plant Staphylococcus isolates identified by the phenotype-based system VITEK 2 (bioMérieux). This commercial kit failed to identify 8 (21%) of the clinical isolates and 32 (84%) of the food and food plant isolates. In contrast, the oligonucleotide array we designed provided an accurate and rapid method for the identification of staphylococcal strains, isolated from clinical, environmental, or food samples, at species level.
葡萄球菌属由36个已确认的物种组成，这些物种包含致病、腐生或用作食品工业发酵剂的菌株。为克服传统鉴定方法的缺点，开发了一种靶向锰依赖性超氧化物歧化酶（sodA）基因的寡核苷酸阵列。利用sodA基因的差异设计寡核苷酸探针，我们发现36个物种中的每一个都有独特的杂交模式。为评估该阵列，我们分析了38株临床分离株以及38株通过基于表型的VITEK 2系统（生物梅里埃公司）鉴定的食品或食品植物葡萄球菌分离株。这种商业试剂盒未能鉴定出8株（21%）临床分离株以及32株（84%）食品和食品植物分离株。相比之下，我们设计的寡核苷酸阵列提供了一种准确、快速的方法，可在物种水平上鉴定从临床、环境或食品样本中分离出的葡萄球菌菌株。
