Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, Gronostajowa 7, 30-387 Krakow, Poland.
MML Centre, Bagno 2, 00-112 Warsaw, Poland.
Int J Mol Sci. 2022 Jun 9;23(12):6450. doi: 10.3390/ijms23126450.
Staphylococcus lugdunensis is an opportunistic pathogen found in the healthy human skin microbiome bacterial community that is able to cause infections of diverse localization, manifestation, and course, including laryngological infections, such as necrotizing sinusitis. Chronic maxillary sinusitis is a disease present in up to one third of European and American populations, and its etiology is not fully described. Within this study, we aimed to characterize 18 S. lugdunensis strains recovered from maxillary sinuses and evaluate them as etiological agents of chronic disease. We performed MLST analysis, the complex analysis of both phenotypic and genetic virulence factors, antibiotic susceptibility profiles, and biofilm formation assay for the detection of biofilm-associated genes. Altogether, S. lugdunensis strains were clustered into eight different STs, and we demonstrated several virulence factors associated with the chronic disease. All tested strains were able to produce biofilm in vitro with numerous strains with a very strong ability, and overall, they were mostly susceptible to antibiotics, although we found resistance to fosfomycin, erythromycin, and clindamycin in several strains. We believe that further in-depth analysis of S. lugdunensis strains from different niches, including the nasal one, should be performed in the future in order to reduce infection rate and broaden the knowledge about this opportunistic pathogen that is gaining attention.
路邓葡萄球菌是一种存在于健康人体皮肤微生物群落中的机会性病原体，能够引起不同定位、表现和病程的感染，包括喉科感染，如坏死性鼻窦炎。慢性上颌窦炎是一种在欧洲和美洲人群中高达三分之一存在的疾病，其病因尚未完全描述。在本研究中，我们旨在对从上颌窦中分离出的 18 株路邓葡萄球菌进行表征，并评估其作为慢性疾病的病因。我们进行了 MLST 分析、表型和遗传毒力因子的复杂分析、抗生素药敏谱分析以及生物膜形成试验，以检测与生物膜相关的基因。总共，路邓葡萄球菌菌株被聚类为八个不同的 ST 型，我们证明了与慢性疾病相关的几种毒力因子。所有测试的菌株都能够在体外形成生物膜，其中许多菌株具有很强的能力，总体而言，它们对大多数抗生素敏感，尽管我们在几个菌株中发现了对磷霉素、红霉素和克林霉素的耐药性。我们认为，未来应该对不同生态位的路邓葡萄球菌菌株进行更深入的分析，包括鼻腔菌株，以降低感染率，并扩大对这种日益受到关注的机会性病原体的认识。
