Institute for Sustainable Agriculture, CSIC, Cordoba, Spain.
J Agric Food Chem. 2010 May 12;58(9):5698-707. doi: 10.1021/jf100215t.
Nitrogen nutrition is one of the major factors limiting the growth and production of crop plants. Limited information on proteome changes occurring in response to nitrogen amount have been available up to now. We used 2-DE to investigate proteome differences between two triticale varieties and the changes caused by nitrogen nutrition deficit in the flag leaf tissue. Some physiological features, such as the number of tillers per plant, SPAD index, dry weight, and protein content were measured previous to the proteomic analysis. Statistical analysis identified 29 differential protein spots in the selected pairwise comparisons of experimental conditions and correlated with the expression cluster revealed by the principal component analysis. The 29 protein spots were subjected to matrix-assisted laser desorption ionization time of flight (MALDI-TOF) to deduce their possible functions. Many of these changes referred to enzymes involved in photosynthesis, metabolic pathways implicated in the balance of the energy, and redox status of the cell. This work provides a first characterization of the proteome changes that occur in response to nitrogen deficit in flag leaves of triticale plants.
氮素营养是限制作物生长和产量的主要因素之一。到目前为止，关于氮素数量响应引起的蛋白质组变化的信息有限。我们使用 2-DE 技术研究了两个黑小麦品种之间的蛋白质组差异，以及氮素营养缺乏对旗叶组织引起的变化。在进行蛋白质组学分析之前，我们测量了一些生理特征，如每株分蘖数、SPAD 指数、干重和蛋白质含量。统计分析鉴定了实验条件的两两比较中 29 个差异蛋白斑点，并与主成分分析揭示的表达簇相关联。对 29 个蛋白斑点进行基质辅助激光解吸电离飞行时间（MALDI-TOF）分析，以推断其可能的功能。这些变化中的许多涉及光合作用中涉及的酶、参与能量平衡和细胞氧化还原状态的代谢途径。这项工作首次描述了氮素缺乏对黑小麦旗叶蛋白质组变化的特征。
