Bohler Sacha, Bagard Matthieu, Oufir Mouhssin, Planchon Sébastien, Hoffmann Lucien, Jolivet Yves, Hausman Jean-François, Dizengremel Pierre, Renaut Jenny
Centre de Recherche Public--Gabriel Lippmann, Département Environnement et Agrobiotechnologies, Belvaux, Luxembourg.
Proteomics. 2007 May;7(10):1584-99. doi: 10.1002/pmic.200600822.
Tropospheric ozone pollution is described as having major negative effects on plants, compromising plant survival. Carbon metabolism is especially affected. In the present work, the effects of chronic ozone exposure were evaluated at the proteomic level in developing leaves of young poplar plants exposed to 120 ppb of ozone for 35 days. Soluble proteins (excluding intrinsic membrane proteins) were extracted from leaves after 3, 14 and 35 days of ozone exposure, as well as 10 days after a recovery period. Proteins (pI 4 to 7) were analyzed by 2-D DIGE experiments, followed by MALDI-TOF-TOF identification. Additional observations were obtained on growth, lesion formation, and leaf pigments analysis. Although treated plants showed large necrotic spots and chlorosis in mature leaves, growth decreased only slightly and plant height was not affected. The number of abscised leaves was higher in treated plants, but new leaf formation was not affected. A decrease in chlorophylls and lutein contents was recorded. A large number of proteins involved in carbon metabolism were identified. In particular, proteins associated with the Calvin cycle and electron transport in the chloroplast were down-regulated. In contrast, proteins associated with glucose catabolism increased in response to ozone exposure. Other identified enzymes are associated with protein folding, nitrogen metabolism and oxidoreductase activity.
对流层臭氧污染被认为对植物有重大负面影响，会危及植物的生存。碳代谢尤其受到影响。在本研究中，在蛋白质组学水平上评估了幼龄杨树发育叶片长期暴露于120 ppb臭氧35天的影响。在臭氧暴露3天、14天和35天后以及恢复期10天后，从叶片中提取可溶性蛋白质（不包括内在膜蛋白）。通过二维差异凝胶电泳实验分析蛋白质（等电点4至7），随后进行基质辅助激光解吸电离飞行时间串联质谱鉴定。还对生长、损伤形成和叶片色素分析进行了额外观察。尽管处理过的植物在成熟叶片上出现了大片坏死斑和黄化现象，但生长仅略有下降，株高未受影响。处理过的植物中脱落叶片的数量较多，但新叶形成未受影响。叶绿素和叶黄素含量有所下降。鉴定出大量参与碳代谢的蛋白质。特别是，与叶绿体中卡尔文循环和电子传递相关的蛋白质被下调。相反，与葡萄糖分解代谢相关的蛋白质在臭氧暴露后增加。其他鉴定出的酶与蛋白质折叠、氮代谢和氧化还原酶活性有关。
