Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej, Frederiksberg, Denmark.
J Agric Food Chem. 2010 Apr 28;58(8):4693-700. doi: 10.1021/jf904362m.
A novel glucosinolate, 3-(hydroxymethyl)pentylglucosinolate, was present at high levels in Cardamine pratensis L. from eastern North America and in commercially obtained seeds, but not in C. pratensis plants from southern Scandinavia. Glucosinolates in a number of accessions of C. pratensis included glucosinolates with the side chains 1-methylethyl, 1-(hydroxymethyl)ethyl, 1-methylpropyl, 1-(hydroxymethyl)propyl, 3-methylpentyl, 3-(hydroxymethyl)pentyl, benzyl, 4-hydroxybenzyl, 4-methoxybenzyl, indol-3-ylmethyl (as well as its 1-methoxy, 4-hydroxy, and 4-methoxy derivatives) and the rare side chain 1,4-dimethoxyindol-3-ylmethyl. Substantial variation was observed for four biosynthetic characters: (i) extent of chain elongation of Ile-derived glucosinolates; (ii) biosynthesis of Phe/Tyr-derived glucosinolates in general; (iii) hydroxylation of branched-chain glucosinolates; and (iv) O-methylation of 4-hydroxybenzylglucosinolate (sinalbin). Cytological analysis of pollen mother cells and root tip cells in meiosis and mitosis established the chromosome number to be 2n = 30 for all accessions, irrespective of glucosinolate profile.
一种新型的硫代葡萄糖苷，3-(羟甲基)戊基硫代葡萄糖苷，在来自北美的东部和商业获得的种子的菘蓝中含量很高，但在来自南斯堪的纳维亚的菘蓝植物中却不存在。几种菘蓝品系中的硫代葡萄糖苷包括具有侧链 1-甲基乙基、1-(羟甲基)乙基、1-甲基丙基、1-(羟甲基)丙基、3-甲基戊基、3-(羟甲基)戊基、苄基、4-羟基苄基、4-甲氧基苄基、吲哚-3-基甲基（以及其 1-甲氧基、4-羟基和 4-甲氧基衍生物）和罕见的侧链 1,4-二甲氧基吲哚-3-基甲基的硫代葡萄糖苷。四个生物合成特征存在很大差异：（i）Ile 衍生的硫代葡萄糖苷的链延长程度；（ii）一般情况下苯丙氨酸/酪氨酸衍生的硫代葡萄糖苷的生物合成；（iii）支链硫代葡萄糖苷的羟化；和（iv）4-羟基苄基硫代葡萄糖苷（萝卜硫素）的 O-甲基化。花粉母细胞和有丝分裂中期和末期根尖细胞的细胞学分析确定所有品系的染色体数为 2n = 30，与硫代葡萄糖苷图谱无关。
