Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China.
Chemosphere. 2010 Apr;79(6):613-9. doi: 10.1016/j.chemosphere.2010.02.045. Epub 2010 Mar 23.
Vigorous anaerobic ammonium oxidation (anammox) activity was realized by seeding with diverse sludge contents. Granules taken from a 2-year old methanogenic reactor loaded with high organic and methanol contents were used as inoculum to enrich anammox biomass in a 1.1L upflow anaerobic sludge blanket reactor. Anammox activity appeared after an operation of 83 d resulting in the final nitrogen removal rate of 11.7 kg N m(-3) d(-1) with the efficient granulation of anammox sludge. The analysis based on 16S rDNA sequencing confirmed that Candidatus "Brocadia" was the dominant population in the enriched biomass.
采用不同的接种污泥量实现了活性很强的厌氧氨氧化（anammox）作用。从一个以高有机物和甲醇含量为负荷的产甲烷反应器中取出的颗粒作为接种物，用于在一个 1.1L 上流式厌氧污泥床反应器中富集厌氧氨氧化生物量。经过 83 天的运行后出现了厌氧氨氧化活性，最终的氮去除率为 11.7kgN m(-3) d(-1)，同时厌氧氨氧化污泥的高效颗粒化。基于 16S rDNA 测序的分析证实，“Brocadia”是富集生物量中的优势种群。
