Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
DNA Repair (Amst). 2010 May 4;9(5):593-6. doi: 10.1016/j.dnarep.2010.02.015. Epub 2010 Mar 23.
Damage detection during nucleotide excision repair requires the action of multiple proteins that probe the DNA for different parameters like disruption of basepairing, DNA bendability and presence of chemical modifications. In a recent study it has been shown that two of these probing events can be spatially separated on the DNA. Upon initial binding of the XPC protein to a region with disrupted basepairing a complex of XPC, TFIIH and XPA is translocated to a CPD lesion even when this chemical modification is located up to 160 nucleotides from the mispaired region.
在核苷酸切除修复过程中进行损伤检测需要多种蛋白质的共同作用，这些蛋白质会探测 DNA 的不同参数，如碱基对的破坏、DNA 的柔韧性和化学修饰的存在。在最近的一项研究中，已经表明这两个探测事件可以在 DNA 上进行空间分离。当 XPC 蛋白最初与碱基对破坏区域结合时，即使该化学修饰位于错配区域 160 个核苷酸之外，XPC、TFIIH 和 XPA 的复合物也会转移到 CPD 损伤处。
