Department of Chemical Engineering, Columbia University in the City of New York, New York, New York 10027, USA.
ASAIO J. 2010 May-Jun;56(3):151-6. doi: 10.1097/MAT.0b013e3181d4ed0f.
This work aimed to demonstrate a new method to determine diffusivities in blood and to show how urea transport is affected by blood cells. Diffusivities of urea in suspensions of bovine erythrocytes in bovine albumin solutions were determined in a two-layer membraneless microfluidic device as a function of interfacial shear rate and hematocrit. The experiments validated the measurement system at zero hematocrit and provided measurements at finite hematocrits, unobtainable in static systems. Both obstruction of diffusion by unsheared and thus non-rotating cells and augmentation of diffusion by cells rotating in response to shear were demonstrated.
这项工作旨在展示一种新的方法来确定血液中的扩散系数，并展示尿素运输如何受到血细胞的影响。在无膜双层微流控装置中，通过界面剪切率和红细胞压积来确定牛血清白蛋白溶液中牛红细胞悬浮液中尿素的扩散系数。实验在零红细胞压积下验证了测量系统，并提供了在静态系统中无法获得的有限红细胞压积下的测量结果。实验证明了未剪切的、因此不旋转的细胞对扩散的阻碍作用，以及细胞对剪切的响应而旋转对扩散的增强作用。
