Giddings J C, Yang F J, Myers M N
J Virol. 1977 Jan;21(1):131-8. doi: 10.1128/JVI.21.1.131-138.1977.
The nature and theory of flow field-flow fractionation is described, and its potential applicability to virus-like particles is discussed. Different virus types are shown to be retained at different levels. Retention can be controlled by variation of the experimental parameters, in good agreement with theory. However, a mild adsorption effect is indicated and requires the development of alternate strategies for measuring diffusion coefficients. For Qbeta, our value agrees well within 10% of literature values; the values obtained for other viruses, using Abeta as an internal standard, are untested. Finally, it is demonstrated that flow field-flow fractionation can cleanly fractionate two viruses from one another and from an albumin impurity, that samples as large as several milligrams in size can be analyzed, and that the method has potential utility in the quantitative and qualitative analysis of virus systems.
描述了流场-流分馏的性质和理论，并讨论了其对病毒样颗粒的潜在适用性。不同病毒类型在不同程度上被保留。保留可以通过实验参数的变化来控制，这与理论高度吻合。然而，表明存在轻微的吸附效应，这需要开发测量扩散系数的替代策略。对于Qβ噬菌体，我们的值与文献值在10%以内吻合良好；使用β淀粉样蛋白作为内标获得的其他病毒的值尚未经过测试。最后，证明了流场-流分馏可以将两种病毒彼此以及与白蛋白杂质干净地分离，能够分析大小达几毫克的样品，并且该方法在病毒系统的定量和定性分析中具有潜在用途。
