Need J T, Butler J F, Zam S G, Wozniak E J
Department of Entomology and Nematology, University of Florida, Gainesville 32611.
J Med Entomol. 1991 Jan;28(1):105-10. doi: 10.1093/jmedent/28.1.105.
The host antibody response of CD1 mice resulting from sequential exposures to two species of Ornithodoros ticks was isotyped (ELISA) in an effort to determine the mechanism for the development of acquired resistance. In addition, salivary gland proteins from two argasid and one ixodid species were examined (RIA) for cross-reactivity. Significant antibody responses, detectable for at least 90 d after last exposure, were shown to occur. Isotyping showed that the initial response was of the IgM class with a secondary class switch to the IgG1 subclass occurring. Evidence that cross-reactive proteins exist between argasid and ixodid ticks is also discussed.
为了确定获得性抗性产生的机制，对连续暴露于两种钝缘蜱的CD1小鼠的宿主抗体反应进行了同型分型（酶联免疫吸附测定）。此外，还对两种软蜱和一种硬蜱的唾液腺蛋白进行了交叉反应性检测（放射免疫分析）。结果显示，在最后一次暴露后至少90天可检测到显著的抗体反应。同型分型表明，初始反应为IgM类，随后发生向IgG1亚类的二次类别转换。文中还讨论了软蜱和硬蜱之间存在交叉反应蛋白的证据。
