Department of Pharmaceutical Sciences, University of Salerno, Fisciano (SA), Italy.
Molecules. 2010 Mar 23;15(3):2028-38. doi: 10.3390/molecules15032028.
This study reports the synthesis and antioxidant activity of some new acetamide derivatives. The compounds' structures were elucidated by NMR analysis and their melting points were measured. The in vitro antioxidant activity of these compounds was tested by evaluating the amount of scavenged ABTS radical and estimating ROS and NO production in tBOH- or LPS-stimulated J774.A1 macrophages. All compounds were tested for their effect on cell viability by an MTT assay and by a Brine Shrimp Test.
本研究报告了一些新的乙酰胺衍生物的合成和抗氧化活性。通过 NMR 分析阐明了化合物的结构，并测量了它们的熔点。通过评估 ABTS 自由基的清除量以及在 tBOH 或 LPS 刺激的 J774.A1 巨噬细胞中测定 ROS 和 NO 的产生来测试这些化合物的体外抗氧化活性。通过 MTT 测定和盐水虾试验测试所有化合物对细胞活力的影响。
