吸入性麻醉剂的光标记
Inhalational anesthetic photolabeling.
作者信息
Eckenhoff Roderic G, Xi Jin, Dailey William P
机构信息
Department of Anesthesiology and Critical Care, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
出版信息
Methods Mol Biol. 2010;617:437-43. doi: 10.1007/978-1-60327-323-7_31.
Abstract
Photolabeling has allowed considerable progress in the understanding of anesthetic binding to proteins, of target identity, and of site localization. There are, however, few groups doing this work, so this article is an attempt to demystify the method. We will discuss the theory, method, and limitations of this useful experimental approach.
摘要
光标记技术在理解麻醉剂与蛋白质的结合、靶点识别以及位点定位方面取得了显著进展。然而，从事这项工作的研究团队较少，因此本文旨在揭开该方法的神秘面纱。我们将讨论这种实用实验方法的理论、方法及局限性。
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本文引用的文献
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Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions.卤代醚麻醉剂的光活性类似物通过低亲和力相互作用提供高分辨率特征。
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