Division of Toxicology and Biomonitoring, Department of Zoology, Shree Shivaji Arts, Commerce and Science College, Rajura, 442905, India.
Fish Physiol Biochem. 2010 Dec;36(4):1145-9. doi: 10.1007/s10695-010-9392-9. Epub 2010 Mar 25.
Liver is the metabolic factory and contains several valuable enzymes that catalyze biochemical reactions. β-glucuronidase is one of the well-known lysozymes that participates in the carbohydrate metabolism in the tissues of various vertebrates. In the present study, an attempt was made to study the kinetic properties of hepatic β-glucuronidase of the Indian major carp (IMC), Labeo rohita. It was observed that the enzyme activity increased largely at pH 5 (0.1 M acetate buffer) when exposed to 38°C. However, the maximum activity was noticed at 52°C and later it started declined up to 70°C. It was also observed that with time the enzyme activity increased until substrate was completely used up. It has been concluded that it is a heat stable enzyme and cannot be destroyed at room temperature. Enzyme activity was observed to increase in response to increase in enzyme and substrate concentrations. The reaction velocity maxima (Vmax) and Michaelis constant (Km) were recorded using Lineweaver-Burk plot that was 18.182 μg/h and 2.907 mM, respectively.
肝脏是代谢工厂，含有几种有价值的酶，这些酶能催化生化反应。β-葡糖苷酸酶是一种众所周知的溶菌酶，参与各种脊椎动物组织中的碳水化合物代谢。在本研究中，尝试研究了印度鲤鱼（Labeo rohita）肝脏β-葡糖苷酸酶的动力学特性。结果表明，当暴露于 38°C 时，该酶在 pH5（0.1 M 醋酸盐缓冲液）时的活性大大增加。然而，在 52°C 时观察到最大活性，随后活性开始下降至 70°C。还观察到，随着时间的推移，酶活性增加，直到底物完全被消耗。结论是，它是一种热稳定的酶，在室温下不会被破坏。酶活性随着酶和底物浓度的增加而增加。通过 Lineweaver-Burk 作图记录了反应速度最大值（Vmax）和米氏常数（Km），分别为 18.182μg/h 和 2.907mM。
