Sarid J
Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
J Neurosci Res. 1991 Feb;28(2):217-28. doi: 10.1002/jnr.490280209.
Developmental regulation of astrocyte-specific expression of the glial fibrillary acidic protein (GFAP) gene reflects transition of immature glioblasts to mature astrocytes. Described here is the cloning and sequencing of the 5'-flanking region of the mouse GFAP gene. It contains a glial-specific positive cis-acting regulatory element that directs preferential expression of a linked reporter gene when transfected into GFAP-positive glioblastoma cells. Sequence analysis of this region revealed the presence of a putative AP-1 binding site, implying a possible role for AP-1 factors in the astroglial-specific expression of the GFAP gene.
胶质纤维酸性蛋白（GFAP）基因在星形胶质细胞中的特异性表达受发育调控，这反映了未成熟成胶质细胞向成熟星形胶质细胞的转变。本文描述了小鼠GFAP基因5'侧翼区的克隆和测序。它包含一个胶质细胞特异性的正向顺式作用调控元件，当转染到GFAP阳性的成胶质细胞瘤细胞中时，该元件可指导相连报告基因的优先表达。对该区域的序列分析揭示了一个假定的AP-1结合位点的存在，这意味着AP-1因子可能在GFAP基因的星形胶质细胞特异性表达中发挥作用。
